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Inhibitory Effects of AF-343, a Mixture of Cassia tora L., Ulmus pumila L., and Taraxacum officinale, on Compound 48/80-Mediated Allergic Responses in RBL-2H3 Cells

1
Bioanalytical and Immunoanalytical Research Group, Korea Institute of Toxicology, 141 Gajungro, Yuseong-gu, Daejeon 34114, Korea
2
Animal Model Research Group, Korea Institute of Toxicology, Jeongeup, Jeonbuk 580-185, Korea
3
Jeonbuk Analytical Research Group, Korea Institute of Toxicology, Jeongeup, Jeonbuk 580-185, Korea
4
Sungkyun Biotech Co., Ltd., SungKyunKwan University, #2066, Seobu-ro, Jangan-gu, Suwon-si, Gyeonggi-do 61256, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Molecules 2020, 25(10), 2434; https://doi.org/10.3390/molecules25102434
Received: 10 April 2020 / Revised: 14 May 2020 / Accepted: 17 May 2020 / Published: 22 May 2020
The purpose of this study was to determine the antiallergic effects of AF-343, a mixture of natural plant extracts from Cassia tora L., Ulmus pumila L., and Taraxacum officinale, on rat basophilic leukemia (RBL-2H3) cells. The inhibitory effects on cell degranulation, proinflammatory cytokine secretion, and reactive oxygen species (ROS) production were studied in compound 48/80-treated RBL-2H3 cells. The bioactive compounds in AF-343 were also identified by HPLC–UV. AF-343 was found to effectively suppress compound 48/80-induced β-hexosaminidase release, and interleukin (IL)-4 and tumor necrosis factor-α (TNF-α) production in RBL-2H3 cells. In addition, AF-343 exhibited DPPH free radical scavenging effects in vitro (half-maximal inhibitory concentration (IC50) = 105 μg/mL) and potently inhibited compound 48/80-induced cellular ROS generation in a 2′,7′-dichlorofluorescein diacetate (DCFH-DA) assay. Specifically, treatment with AF-343 exerted stronger antioxidant effects in vitro and antiallergic effects in cells than treatment with three single natural plant extracts. Furthermore, AF-343 was observed to contain bioactive compounds, including catechin, aurantio-obtusin, and chicoric acid, which have been reported to elicit antiallergic responses. This study reveals that AF-343 attenuates allergic responses via suppression of β-hexosaminidase release, IL-4 and TNF-α secretion, and ROS generation, perhaps through mechanisms related to catechin, aurantio-obtusin, and chicoric acid. The results indicate that AF-343 can be considered a treatment for various allergic diseases. View Full-Text
Keywords: AF-343; Cassia tora L., Ulmus pumila L., Taraxacum officinale; antiallergic responses; compound 48/80; RBL-2H3 cells AF-343; Cassia tora L., Ulmus pumila L., Taraxacum officinale; antiallergic responses; compound 48/80; RBL-2H3 cells
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Lee, E.K.; Song, J.; Seo, Y.; Koh, E.M.; Kim, S.-H.; Jung, K.J. Inhibitory Effects of AF-343, a Mixture of Cassia tora L., Ulmus pumila L., and Taraxacum officinale, on Compound 48/80-Mediated Allergic Responses in RBL-2H3 Cells. Molecules 2020, 25, 2434.

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