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Isolation and Characterization of a Novel Sialoglycopeptide Promoting Osteogenesis from Gadus morhua Eggs

by 1,2,†, 1,2,†, 3,†, 4, 1,2, 1,2,* and 5,*
1
Hainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, Hainan University, Hainan 570228, China
2
College of Food Science and Technology, Hainan University, Hainan 570228, China
3
Marine Biomedical Research Institute of Qingdao, Qingdao 266003, China
4
Hainan Institute for Food Control, Hainan 570228, China
5
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this study.
Molecules 2020, 25(1), 156; https://doi.org/10.3390/molecules25010156
Received: 13 November 2019 / Revised: 23 December 2019 / Accepted: 27 December 2019 / Published: 30 December 2019
Gadus morhua eggs contain several nutrients, including polyunsaturated fatty acids, lecithin and glycoproteins. A novel sialoglycopeptide from the eggs of G. morhua (Gm-SGPP) was extracted with 90% phenol and purified by Q Sepharose Fast Flow (QFF) ion exchange chromatography, followed by S-300 gel filtration chromatography. Gm-SGPP contained 63.7% carbohydrate, 16.2% protein and 18.6% N-acetylneuraminic acid. High-performance size exclusion chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that Gm-SGPP is a 7000-Da pure sialoglycopeptide. β-elimination reaction suggested that Gm-SGPP contained N-glycan units. Amino acid N-terminal sequence analysis indicated the presence of Ala-Ser-Asn-Gly-Thr-Gln-Ala-Pro amino acid sequence. Moreover, N-glycan was connected at the third Asn location of the peptide chain through GlcNAc. Gm-SGPP was composed of D-mannose, D-glucuronic acid and D-galactose. Fourier transform-infrared spectroscopy (FT-IR), 1H-nuclear magnetic resonance spectroscopy (1H-NMR) and methylation analysis were performed to reveal the structure profile of Gm-SGPP. In vitro results showed that the proliferation activity of MC3T3-E1 cells was significantly promoted by Gm-SGPP. In vivo data revealed that Gm-SGPP increased the calcium and phosphorus content of tibias and promoted longitudinal bone growth in adolescent rats. View Full-Text
Keywords: Gadus morhua eggs; sialoglycopeptide; osteogenesis; structure characterization Gadus morhua eggs; sialoglycopeptide; osteogenesis; structure characterization
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MDPI and ACS Style

Hei, Z.; Zhao, M.; Tian, Y.; Chang, H.; Shen, X.; Xia, G.; Wang, J. Isolation and Characterization of a Novel Sialoglycopeptide Promoting Osteogenesis from Gadus morhua Eggs. Molecules 2020, 25, 156. https://doi.org/10.3390/molecules25010156

AMA Style

Hei Z, Zhao M, Tian Y, Chang H, Shen X, Xia G, Wang J. Isolation and Characterization of a Novel Sialoglycopeptide Promoting Osteogenesis from Gadus morhua Eggs. Molecules. 2020; 25(1):156. https://doi.org/10.3390/molecules25010156

Chicago/Turabian Style

Hei, Zhiliang; Zhao, Meihui; Tian, Yingying; Chang, Hong; Shen, Xuanri; Xia, Guanghua; Wang, Jingfeng. 2020. "Isolation and Characterization of a Novel Sialoglycopeptide Promoting Osteogenesis from Gadus morhua Eggs" Molecules 25, no. 1: 156. https://doi.org/10.3390/molecules25010156

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