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HBX-6, Standardized Cornus officinalis and Psoralea corylifolia L. Extracts, Suppresses Benign Prostate Hyperplasia by Attenuating E2F1 Activation

1
Department of Pharmacology, College of Korean Medicine, Sangji University, 83 Sangjidae-gil, Wonju-si, Gangwon-do 26339, Korea
2
Central Research Institue of Hawon Pharmaceutical, Jangheung 59338, Korea
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(9), 1719; https://doi.org/10.3390/molecules24091719
Received: 28 March 2019 / Revised: 27 April 2019 / Accepted: 28 April 2019 / Published: 2 May 2019
Background: The aim of this study was to simplify and identify the contents of the herbal formula, HBX-5. This study was carried out to evaluate the therapeutic effects of HBX-6 in a mouse model of benign prostatic hyperplasia (BPH). Based on in vitro, we selected a candidate, reconstituted an experimental agent and investigated the effects on testosterone-induced BPH rats. Cell viability was determined by MTT assay in RWPE-1 and WPMY-1 cells. The expression of androgen receptor (AR) was measured in dihydrotestosterone-stimulated RWPE-1 and WPMY-1 cells. BPH was induced in mice by a subcutaneous injection of testosterone propionate for four weeks. Animals were divided into six groups: Group 1, control mice; Group 2, mice with BPH; Group 3, mice with BPH treated with finasteride; Group 4, mice with BPH treated with 200 mg/kg HBX-5; Group 5, mice with BPH treated with 100 mg/kg HBX-6; and Group 6, mice with BPH treated with 200 mg/kg HBX-6. Changes in prostate weight were measured after treatments, and the thickness of the epithelium was evaluated. The expression levels of proteins associated with prostatic cell proliferation and cell cycle-related proteins were determined. Based on previous reports and in vitro results, we selected Cornus officinalis and Psoralea corylifolia among HBX-5 components and reconstituted the experimental agent, and named it HBX-6. The result represented a new herbal formula, HBX-6 that suppressed the pathological alterations in BPH and showed a marked reduction in proliferation-related protein expression compared to mice with BPH. Our results indicate that HBX-6 has a better therapeutic effect in the BPH murine model than those of HBX-5 and finasteride, suggesting the role of HBX-6 as a new BPH remedial agent. View Full-Text
Keywords: BPH; RWPE-1; WPMY-1; E2F1; Cornus officinalis Sieb. et Zucc.; Psoralea corylifolia L.; HBX-6 BPH; RWPE-1; WPMY-1; E2F1; Cornus officinalis Sieb. et Zucc.; Psoralea corylifolia L.; HBX-6
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MDPI and ACS Style

Jin, B.-R.; Kim, H.-J.; Seo, J.-H.; Kim, M.-S.; Lee, K.-H.; Yoon, I.-J.; An, H.-J. HBX-6, Standardized Cornus officinalis and Psoralea corylifolia L. Extracts, Suppresses Benign Prostate Hyperplasia by Attenuating E2F1 Activation. Molecules 2019, 24, 1719. https://doi.org/10.3390/molecules24091719

AMA Style

Jin B-R, Kim H-J, Seo J-H, Kim M-S, Lee K-H, Yoon I-J, An H-J. HBX-6, Standardized Cornus officinalis and Psoralea corylifolia L. Extracts, Suppresses Benign Prostate Hyperplasia by Attenuating E2F1 Activation. Molecules. 2019; 24(9):1719. https://doi.org/10.3390/molecules24091719

Chicago/Turabian Style

Jin, Bo-Ram, Hyo-Jung Kim, Jong-Hwan Seo, Myoung-Seok Kim, Kwang-Ho Lee, Il-Joo Yoon, and Hyo-Jin An. 2019. "HBX-6, Standardized Cornus officinalis and Psoralea corylifolia L. Extracts, Suppresses Benign Prostate Hyperplasia by Attenuating E2F1 Activation" Molecules 24, no. 9: 1719. https://doi.org/10.3390/molecules24091719

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