Next Article in Journal
Modelling Contaminant Formation during Thermal Processing of Sea Buckthorn Purée
Next Article in Special Issue
p53 Binds Preferentially to Non-B DNA Structures Formed by the Pyrimidine-Rich Strands of GAA·TTC Trinucleotide Repeats Associated with Friedreich’s Ataxia
Previous Article in Journal
Copper-Catalyzed Regioselective Synthesis of (E)-β-Fluorovinyl Sulfones
Article Menu
Issue 8 (April-2) cover image

Export Article

Open AccessArticle

Impact of Small Molecules on Intermolecular G-Quadruplex Formation

1
Department of Physics, Kent State University, Kent, OH 44242, USA
2
Department of Chemistry and Biochemistry, Kent State University, Kent, OH 44242, USA
3
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Liliya Yatsunyk and David Monchaud
Molecules 2019, 24(8), 1570; https://doi.org/10.3390/molecules24081570
Received: 9 February 2019 / Revised: 18 April 2019 / Accepted: 19 April 2019 / Published: 20 April 2019
(This article belongs to the Special Issue Recent Advances in Non-Canonical Nucleic Acid Structures)
  |  
PDF [1245 KB, uploaded 20 April 2019]
  |     |  

Abstract

We performed single molecule studies to investigate the impact of several prominent small molecules (the oxazole telomestatin derivative L2H2-6OTD, pyridostatin, and Phen-DC3) on intermolecular G-quadruplex (i-GQ) formation between two guanine-rich DNA strands that had 3-GGG repeats in one strand and 1-GGG repeat in the other (3+1 GGG), or 2-GGG repeats in each strand (2+2 GGG). Such structures are not only physiologically significant but have recently found use in various biotechnology applications, ranging from DNA-based wires to chemical sensors. Understanding the extent of stability imparted by small molecules on i-GQ structures, has implications for these applications. The small molecules resulted in different levels of enhancement in i-GQ formation, depending on the small molecule and arrangement of GGG repeats. The largest enhancement we observed was in the 3+1 GGG arrangement, where i-GQ formation increased by an order of magnitude, in the presence of L2H2-6OTD. On the other hand, the enhancement was limited to three-fold with Pyridostatin (PDS) or less for the other small molecules in the 2+2 GGG repeat case. By demonstrating detection of i-GQ formation at the single molecule level, our studies illustrate the feasibility to develop more sensitive sensors that could operate with limited quantities of materials. View Full-Text
Keywords: G-quadruplex; small molecule; single molecule; FRET; biosensor G-quadruplex; small molecule; single molecule; FRET; biosensor
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Gyawali, P.; GC, K.; Ma, Y.; Abeysirigunawardena, S.; Nagasawa, K.; Balci, H. Impact of Small Molecules on Intermolecular G-Quadruplex Formation. Molecules 2019, 24, 1570.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top