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Molecules 2019, 24(8), 1521; https://doi.org/10.3390/molecules24081521 (registering DOI)

Quality Evaluation of Gastrodia Elata Tubers Based on HPLC Fingerprint Analyses and Quantitative Analysis of Multi-Components by Single Marker

2,†, 1,†, 1, 1, 1,3,4 and 1,3,4,*
1
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
2
School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China
3
Yunnan Key Laboratory of Panax notoginseng, Kunming University of Science and Technology, Kunming 650500, China
4
Institute of Biology Leiden, Leiden University, 2333BE Leiden, The Netherlands
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Marcello Locatelli, Angela Tartaglia, Dora Melucci, Abuzar Kabir, Halil Ibrahim Ulusoy and Victoria Samanidou
Received: 24 February 2019 / Revised: 13 April 2019 / Accepted: 15 April 2019 / Published: 17 April 2019
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Abstract

Gastrodia elata (G. elata) tuber is a valuable herbal medicine used to treat many diseases. The procedure of establishing a reasonable and feasible quality assessment method for G. elata tuber is important to ensure its clinical safety and efficacy. In this research, an effective and comprehensive evaluation method for assessing the quality of G. elata has been developed, based on the analysis of high performance liquid chromatography (HPLC) fingerprint, combined with the quantitative analysis of multi-components by single marker (QAMS) method. The contents of the seven components, including gastrodin, p-hydroxybenzyl alcohol, p-hydroxy benzaldehyde, parishin A, parishin B, parishin C, and parishin E were determined, simultaneously, using gastrodin as the reference standard. The results demonstrated that there was no significant difference between the QAMS method and the traditional external standard method (ESM) (p > 0.05, RSD < 4.79%), suggesting that QAMS was a reliable and convenient method for the content determination of multiple components, especially when there is a shortage of reference substances. In conclusion, this strategy could be beneficial for simplifying the processes in the quality control of G. elata tuber and giving references to promote the quality standards of herbal medicines. View Full-Text
Keywords: Gastrodia elata tuber; quality evaluation; HPLC; QAMS Gastrodia elata tuber; quality evaluation; HPLC; QAMS
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Li, Y.; Zhang, Y.; Zhang, Z.; Hu, Y.; Cui, X.; Xiong, Y. Quality Evaluation of Gastrodia Elata Tubers Based on HPLC Fingerprint Analyses and Quantitative Analysis of Multi-Components by Single Marker. Molecules 2019, 24, 1521.

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