Next Article in Journal
Chemicals from Food Supply Chain By-Products and Waste Streams
Next Article in Special Issue
Reduction of Preneoplastic Lesions Induced by 1,2-Dimethylhydrazine in Rat Colon by Maslinic Acid, a Pentacyclic Triterpene from Olea europaea L.
Previous Article in Journal
Enhanced Supercapacitor Performance Based on CoAl Layered Double Hydroxide-Polyaniline Hybrid Electrodes Manufactured Using Hydrothermal-Electrodeposition Technology
Previous Article in Special Issue
Triterpene-Based Carboxamides Act as Good Inhibitors of Butyrylcholinesterase
Article Menu
Issue 5 (March-1) cover image

Export Article

Open AccessArticle

Identification and Characterization of the Caspase-Mediated Apoptotic Activity of Teucrium mascatense and an Isolated Compound in Human Cancer Cells

1
Department of Biochemistry, College of Medicine & Health Sciences, United Arab Emirates (UAE) University, Al Ain 20000, UAE
2
Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, Oman
3
Department of Biological Sciences & Chemistry, College of Arts and Sciences, University of Nizwa, Nizwa, Oman
*
Author to whom correspondence should be addressed.
These authors contributed equally to this project.
Academic Editors: Eva E. Rufino-Palomares and José Antonio Lupiáñez
Molecules 2019, 24(5), 977; https://doi.org/10.3390/molecules24050977
Received: 16 January 2019 / Revised: 3 March 2019 / Accepted: 4 March 2019 / Published: 11 March 2019
(This article belongs to the Special Issue Natural Products and Derivatives in Human Disorders)
  |  
PDF [11944 KB, uploaded 11 March 2019]
  |  

Abstract

Plants of the genus Teucrium (Lamiaceae or Labiatae family) are known historically for their medicinal value. Here, we identify and characterize the anticancer potential of T. mascatense and its active compound, IM60, in human cancer cells. The anti-proliferative effect of a T. mascatense methanol extract and its various fractions were analyzed in MCF-7 and HeLa cells in a dose- and time dependent manner. The dichloromethane fraction (TMDF) was observed to be the most effective with cytotoxicity against a more expanded series of cell lines, including MDA-MB-231. A time and dose-dependent toxicity profile was also observed for IM60; it could induce rapid cell death (within 3 h) in MCF-7 cells. Activation of caspases and PARP, hallmarks of apoptotic cell death pathways, following treatment with TMDF was demonstrated using western blot analysis. Inversion of the phosphatidylserine phospholipid from the inner to the outer membrane was confirmed by annexin V staining that was inhibited by the classical apoptosis inhibitor, Z-VAK-FMK. Changes in cell rounding, shrinkage, and detachment from other cells following treatment with TMDF and IM60 also supported these findings. Finally, the potential of TMDF and IM60 to induce enzymatic activity of caspases was also demonstrated in MCF-7 cells. This study, thus, not only characterizes the anticancer potential of T. mascatense, but also identifies a lead terpenoid, IM60, with the potential to activate anticancer cell death pathways in human cancer cells. View Full-Text
Keywords: Teucrium mascatense; natural plant products; anticancer activity; breast and cervical cancer; apoptosis; caspases Teucrium mascatense; natural plant products; anticancer activity; breast and cervical cancer; apoptosis; caspases
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Panicker, N.G.; Balhamar, S.O.M.S.; Akhlaq, S.; Qureshi, M.M.; Rizvi, T.S.; Al-Harrasi, A.; Hussain, J.; Mustafa, F. Identification and Characterization of the Caspase-Mediated Apoptotic Activity of Teucrium mascatense and an Isolated Compound in Human Cancer Cells. Molecules 2019, 24, 977.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top