Next Article in Journal
Multi-Steps Fragmentation-Ion Trap Mass Spectrometry Coupled to Liquid Chromatography Diode Array System for Investigation of Olaparib Related Substances
Previous Article in Journal
Identification of Compounds That Inhibit Estrogen-Related Receptor Alpha Signaling Using High-Throughput Screening Assays
Open AccessArticle

Chemical Diversity of Codium bursa (Olivi) C. Agardh Headspace Compounds, Volatiles, Fatty Acids and Insight into Its Antifungal Activity

1
Faculty of Chemistry and Technology, University of Split, 21000 Split, Croatia
2
Department of Food Technology, University Center Koprivnica, University North, Trg dr. Žarka Dolinara 1, 48000 Koprivnica, Croatia
3
Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, 31000 Osijek, Croatia
4
Croatian Veterinary Institute, Branch-Veterinary Institute Vinkovci, Josipa Kozarca 24, 32100 Vinkovci, Croatia
5
Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
*
Author to whom correspondence should be addressed.
Academic Editors: Jordi Molgó, Olivier P. Thomas and Denis Servent
Molecules 2019, 24(5), 842; https://doi.org/10.3390/molecules24050842
Received: 16 January 2019 / Revised: 22 February 2019 / Accepted: 22 February 2019 / Published: 27 February 2019
The focus of present study is on Codium bursa collected from the Adriatic Sea. C. bursa volatiles were identified by gas chromatography and mass spectrometry (GC-FID; GC-MS) after headspace solid-phase microextraction (HS-SPME), hydrodistillation (HD), and supercritical CO2 extraction (SC-CO2). The headspace composition of dried (HS-D) and fresh (HS-F) C. bursa was remarkably different. Dimethyl sulfide, the major HS-F compound was present in HS-D only as a minor constituent and heptadecane percentage was raised in HS-D. The distillate of fresh C. bursa contained heptadecane and docosane among the major compounds. After air-drying, a significantly different composition of the volatile oil was obtained with (E)-phytol as the predominant compound. It was also found in SC-CO2 extract of freeze-dried C. bursa (FD-CB) as the major constituent. Loliolide (3.51%) was only identified in SC-CO2 extract. Fatty acids were determined from FD-CB after derivatisation as methyl esters by GC-FID. The most dominant acids were palmitic (25.4%), oleic (36.5%), linoleic (11.6%), and stearic (9.0%). FD-CB H2O extract exhibited better antifungal effects against Fusarium spp., while dimethyl sulfoxide (DMSO) extract was better for the inhibition of Penicillium expansum, Aspergillus flavus, and Rhizophus spp. The extracts showed relatively good antifungal activity, especially against P. expansum (for DMSO extract MIC50 was at 50 µg/mL). View Full-Text
Keywords: headspace solid-phase microextraction (HS-SPME); distillation; supercritical CO2 extraction; gas chromatography and mass spectrometry (GC-MS) headspace solid-phase microextraction (HS-SPME); distillation; supercritical CO2 extraction; gas chromatography and mass spectrometry (GC-MS)
Show Figures

Figure 1

MDPI and ACS Style

Jerković, I.; Kranjac, M.; Marijanović, Z.; Šarkanj, B.; Cikoš, A.-M.; Aladić, K.; Pedisić, S.; Jokić, S. Chemical Diversity of Codium bursa (Olivi) C. Agardh Headspace Compounds, Volatiles, Fatty Acids and Insight into Its Antifungal Activity. Molecules 2019, 24, 842.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map

1
Back to TopTop