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Open AccessArticle

GC-MS-Based Metabolomics to Reveal the Protective Effect of Gross Saponins of Tribulus terrestris Fruit against Ischemic Stroke in Rat

1
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China
2
Key Laboratory of Medicinal Materials, Jilin Academy of Chinese Medicine Sciences, Changchun 130021, China
3
Academy of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
*
Authors to whom correspondence should be addressed.
Molecules 2019, 24(4), 793; https://doi.org/10.3390/molecules24040793
Received: 8 January 2019 / Revised: 20 February 2019 / Accepted: 21 February 2019 / Published: 22 February 2019
(This article belongs to the Section Analytical Chemistry)
Stroke is one of the most common neurological disorders and seriously threatens human life. Gross saponins of Tribulus terrestris fruit (GSTTF) are used for neuroprotective treatment on convalescents of ischemic stroke. However, the therapeutic effects and mechanisms have not yet well understood, especially from the metabolic perspective. In this study, the protective effect of GSTTF on ischemic stroke in a middle cerebral artery occlusion (MCAO) rat model was investigated by the GC-MS-based metabolomics approach. 2,3,5-triphenyltetrazolium chloride (TTC) staining of brain tissues showed that GSTTF significantly reduced the infarct area after MCAO surgery. Metabolomic profiling showed a series of metabolic perturbation occurs in ischemic stroke compared with sham group. GSTTF can reverse the MCAO-induced serum metabolic deviations by regulating multiple metabolic pathways including fatty acids metabolism, amino acids metabolism, and carbohydrates metabolism. The current study provided a useful approach for understanding the mechanism of MCAO-induced ischemic stroke and a reliable basis for evaluating the efficacy of GSTTF in the treatment of ischemic stroke. View Full-Text
Keywords: GC-MS; metabolomics; gross saponins of Tribulus terrestris; ischemic stroke GC-MS; metabolomics; gross saponins of Tribulus terrestris; ischemic stroke
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Wang, Y.; Zhao, H.; Liu, Y.; Guo, W.; Bao, Y.; Zhang, M.; Xu, T.; Xie, S.; Liu, X.; Xu, Y. GC-MS-Based Metabolomics to Reveal the Protective Effect of Gross Saponins of Tribulus terrestris Fruit against Ischemic Stroke in Rat. Molecules 2019, 24, 793.

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