Immunomodulatory Effects of the Meretrix Meretrix Oligopeptide (QLNWD) on Immune-Deficient Mice
Abstract
:1. Introduction
2. Results and Discussion
2.1. Comparison of Body Weight
2.2. Thymus and Spleen Indexes
2.3. Morphological Observations of Mouse Organs
2.4. Serum Immunoglobulin G (IgG) Levels
2.5. Serum Hemolysin
2.6. T Lymphocyte Assessment
3. Materials and Methods
3.1. Animals
3.2. Materials and Chemical Reagents
3.3. Animal Groupings and Treatments
3.4. Body Weight and Immune Organ Index Changes
3.5. Histomorphological Observation
3.6. Determination of IgG Serum Content
3.7. Detection of Serum Hemolysin
3.8. Proliferation of Spleen T-Lymphocytes
3.9. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Group | HC50 | Proliferation Rate % |
---|---|---|
Negative Control | 87.58 ± 0.05 | 0 |
Diseased Model | 86.90 ± 0.05 | −0.78 ± 0.05 |
Positive Control | 91.39 ± 0.03 * | 4.35 ± 0.03 * |
Low-dose | 87.84 ± 0.10 | 0.29 ± 0.10 |
Medium-dose | 89.27 ± 0.07 | 1.92 ± 0.07 |
High-dose | 90.78 ± 0.05 * | 3.65 ± 0.05 * |
Group | Pre-Treatment (10 days) | Treatment (5 days) |
---|---|---|
Dose (0.2 mL) | Dose (0.2 mL) | |
Negative Control | NS | NS |
Disease Model | NS | CTX (80 mg/kg BW) |
Positive Control | Levamisole (25 mg/kg BW) | CTX (80 mg/kg BW) |
Low Dose | MMO (50 mg/kg BW) | CTX (80 mg/kg BW) |
Medium Dose | MMO (100 mg/kg BW) | CTX (80 mg/kg BW) |
High Dose | MMO (200 mg/kg BW) | CTX (80 mg/kg BW) |
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Zhang, W.; Ye, L.; Wang, F.; Zheng, J.; Tian, X.; Chen, Y.; Ding, G.; Yang, Z. Immunomodulatory Effects of the Meretrix Meretrix Oligopeptide (QLNWD) on Immune-Deficient Mice. Molecules 2019, 24, 4452. https://doi.org/10.3390/molecules24244452
Zhang W, Ye L, Wang F, Zheng J, Tian X, Chen Y, Ding G, Yang Z. Immunomodulatory Effects of the Meretrix Meretrix Oligopeptide (QLNWD) on Immune-Deficient Mice. Molecules. 2019; 24(24):4452. https://doi.org/10.3390/molecules24244452
Chicago/Turabian StyleZhang, Wen, Lei Ye, Fenglei Wang, Jiawen Zheng, Xiaoxiao Tian, Yan Chen, Guofang Ding, and Zuisu Yang. 2019. "Immunomodulatory Effects of the Meretrix Meretrix Oligopeptide (QLNWD) on Immune-Deficient Mice" Molecules 24, no. 24: 4452. https://doi.org/10.3390/molecules24244452