CX-5461 Inhibits Pancreatic Ductal Adenocarcinoma Cell Growth, Migration and Induces DNA Damage
Abstract
:1. Introduction
2. Results
2.1. CX-5461 Inhibits Pancreatic Cancer Cell Growth in Nanomolar Range
2.2. CX-5461 Inhibits PANC-1 Cell Migration
2.3. CX-5461 Induces mRNA Expression of EMT Markers
2.4. DNA Damage Induced by CX-5461
2.5. The Effect of CX-5461 in 3D Spheroid Models
2.6. Combination of CX-5461 and Gemcitabine Leads to An Antagonistic Effect
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Chemicals
4.3. Sulforhodamine B (SRB) Assay
4.4. Migration Assay
4.5. qRT-PCR
4.6. Immunofluoresent Staining and Imaging
4.7. Western Blot
4.8. Spheroid Formation
4.9. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
Cell Line | Average IC50 (nM) ± SEM | Average Doubling Time in Hours ± SEM |
---|---|---|
SUIT-2-28 | 77 ± 14 | 16.4 ± 0.20 |
PDAC-3 | 114 ± 30 | 18.8 ± 1.36 |
PANC-1 | 199 ± 26 | 28.4 ± 1.91 |
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El Hassouni, B.; Mantini, G.; Immordino, B.; Peters, G.J.; Giovannetti, E. CX-5461 Inhibits Pancreatic Ductal Adenocarcinoma Cell Growth, Migration and Induces DNA Damage. Molecules 2019, 24, 4445. https://doi.org/10.3390/molecules24244445
El Hassouni B, Mantini G, Immordino B, Peters GJ, Giovannetti E. CX-5461 Inhibits Pancreatic Ductal Adenocarcinoma Cell Growth, Migration and Induces DNA Damage. Molecules. 2019; 24(24):4445. https://doi.org/10.3390/molecules24244445
Chicago/Turabian StyleEl Hassouni, Btissame, Giulia Mantini, Benoît Immordino, Godefridus J. Peters, and Elisa Giovannetti. 2019. "CX-5461 Inhibits Pancreatic Ductal Adenocarcinoma Cell Growth, Migration and Induces DNA Damage" Molecules 24, no. 24: 4445. https://doi.org/10.3390/molecules24244445