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Recent Advances in Aggregation-Induced Emission Chemosensors for Anion Sensing
Open AccessFeature PaperArticle

Expanding the Toolbox for Label-Free Enzyme Assays: A Dinuclear Platinum(II) Complex/DNA Ensemble with Switchable Near-IR Emission

Department of Chemistry, University of Iowa, Iowa City, IA 52242, USA
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Author to whom correspondence should be addressed.
Molecules 2019, 24(23), 4390; https://doi.org/10.3390/molecules24234390
Received: 22 October 2019 / Revised: 25 November 2019 / Accepted: 26 November 2019 / Published: 1 December 2019
(This article belongs to the Special Issue Aggregation-Induced Emission: Materials and Applications)
Switchable luminescent bioprobes whose emission can be turned on as a function of specific enzymatic activity are emerging as important tools in chemical biology. We report a promising platform for the development of label-free and continuous enzymatic assays in high-throughput mode based on the reversible solvent-induced self-assembly of a neutral dinuclear Pt(II) complex. To demonstrate the utility of this strategy, the switchable luminescence of a dinuclear Pt(II) complex was utilized in developing an experimentally simple, fast (10 min), low cost, and label-free turn-on luminescence assay for the endonuclease enzyme DNAse I. The complex displays a near-IR (NIR) aggregation-induced emission at 785 nm in aqueous solution that is completely quenched upon binding to G-quadruplex DNA from the human c-myc oncogene. Luminescence is restored upon DNA degradation elicited by exposure to DNAse I. Correlation between near-IR luminescence intensity and DNAse I concentration in human serum samples allows for fast and label-free detection of DNAse I down to 0.002 U/mL. The Pt(II) complex/DNA assembly is also effective for identification of DNAse I inhibitors, and assays can be performed in multiwell plates compatible with high-throughput screening. The combination of sensitivity, speed, convenience, and cost render this method superior to all other reported luminescence-based DNAse I assays. The versatile response of the Pt(II) complex to DNA structures promises broad potential applications in developing real-time and label-free assays for other nucleases as well as enzymes that regulate DNA topology. View Full-Text
Keywords: label-free assay; near-infrared; deoxyribonuclease I; platinum; aggregation-induced emission; supramolecular chemistry label-free assay; near-infrared; deoxyribonuclease I; platinum; aggregation-induced emission; supramolecular chemistry
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MDPI and ACS Style

Gabr, M.T.; Pigge, F.C. Expanding the Toolbox for Label-Free Enzyme Assays: A Dinuclear Platinum(II) Complex/DNA Ensemble with Switchable Near-IR Emission. Molecules 2019, 24, 4390.

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