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Open AccessArticle

Oroxin B Induces Apoptosis by Down-Regulating MicroRNA-221 Resulting in the Inactivation of the PTEN/PI3K/AKT Pathway in Liver Cancer

1
School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116000, China
2
Component Medicine Engineering Research Center of Liaoning Province, Dalian 116000, China
3
Liaoning Province Modern Chinese Medicine Research Engineering Laboratory, Dalian 116000, China
4
Agilent Technologies Modern TCM and Multi-Omics Research Collaboration Lab, Liaoning University of Traditional Chinese Medicine, Dalian 116000, China
*
Author to whom correspondence should be addressed.
Academic Editors: Kamelija Zarkovic and Neven Zarkovic
Molecules 2019, 24(23), 4384; https://doi.org/10.3390/molecules24234384
Received: 16 October 2019 / Revised: 25 November 2019 / Accepted: 28 November 2019 / Published: 30 November 2019
(This article belongs to the Collection Molecular Medicine)
This study aims to investigate the anticancer effect of Oroxin B (OB) both in vitro and in vivo, and the molecular mechanism involved in microRNA-221 and the PI3K/Akt/PTEN pathway through modulation of apoptosis in Hepatocellular carcinoma (HCC). DEN-induced rats and HepG2 cells based on the microfluidic chip were employed, while the mRNA and protein expression of microRNA-221, PI3K, p-Akt and PTEN were evaluated by RT-PCR and Western blot analysis. Based on Microfluidic Chip and DEN-induced rat model, OB effectively exerts anti-liver cancer effect both in vitro and in vivo, and the expression of miR-221 in OB treated groups was significantly lower than that in the control group (** p < 0.01). The RT-PCR and Western blot results suggested the PI3K mRNA and protein in OB treated groups were both lower than those in control group and indicated the overexpression of PTEN. Therefore, OB effectively exerts anticancer effects by positively regulating the PTEN gene and then inactivating the PI3K/Akt signaling pathway through down-regulating the expression of the microRNA-221, thereby inducing apoptosis of liver cancer cells. This study offers a theoretical evidence for further development and clinical guidance of OB as an anti-tumor agent. View Full-Text
Keywords: Oroxin B; liver cancer; Microfluidic Chip; DEN-induced rats model; microRNA-221; PI3K/Akt/PTEN pathway Oroxin B; liver cancer; Microfluidic Chip; DEN-induced rats model; microRNA-221; PI3K/Akt/PTEN pathway
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MDPI and ACS Style

Li, N.; Men, W.; Zheng, Y.; Wang, H.; Meng, X. Oroxin B Induces Apoptosis by Down-Regulating MicroRNA-221 Resulting in the Inactivation of the PTEN/PI3K/AKT Pathway in Liver Cancer. Molecules 2019, 24, 4384.

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