Speciation Studies of Bifunctional 3-Hydroxy-4-Pyridinone Ligands in the Presence of Zn²⁺ at Different Ionic Strengths and Temperatures

The acid–base properties of two bifunctional 3-hydroxy-4-pyridinone ligands and their chelating capacity towards Zn²⁺, an essential bio-metal cation, were investigated in NaCl aqueous solutions by potentiometric, UV-Vis spectrophotometric, and ¹H NMR spectroscopic titrations, carried out at 0.15 ≤ I/mol ⁻¹ ≤ 1.00 and 288.15 ≤ T/K ≤ 310.15. A study at I = 0.15 mol L⁻¹ and T = 298.15 K was also performed for other three Zn²⁺/L^z− systems, with ligands belonging to the same family of compounds. The processing of experimental data allowed the determination of protonation and stability constants, which showed accordance with the data obtained from the different analytical techniques used, and with those reported in the literature for the same class of compounds. ESI-MS spectrometric measurements provided support for the formation of the different Zn²⁺/ligand species, while computational molecular simulations allowed information to be gained on the metal–ligand coordination. The dependence on ionic strength and the temperature of equilibrium constants were investigated by means of the extended Debye–Hückel model, the classical specific ion interaction theory, and the van’t Hoff equations, respectively. View Full-Text