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Open AccessArticle

Andean Prumnopitys Andina (Podocarpacae) Fruit Extracts: Characterization of Secondary Metabolites and Potential Cytoprotective Effect

1
Departamento de Ciencias Básicas Biomédicas, Facultad de Ciencias de la Salud, Universidad de Talca, Talca 3460000, Chile
2
Laboratorio de Cultivo Celular, Facultad de Ciencias de la Salud, Universidad de Talca, Talca 3460000, Chile
3
Laboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales, Universidad de Talca, Talca 3460000, Chile
4
Escuela de Nutrición y Dietética, Facultad de Ciencias de la Salud, Universidad de Talca, Talca 3460000, Chile
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(22), 4028; https://doi.org/10.3390/molecules24224028
Received: 17 October 2019 / Revised: 4 November 2019 / Accepted: 5 November 2019 / Published: 7 November 2019
(This article belongs to the Section Natural Products Chemistry)
The fruits from the Chilean Podocarpaceae Prumnopitys andina have been consumed since pre-Hispanic times. Little is known about the composition and biological properties of this fruit. The aim of this work was to identify the secondary metabolites of the edible part of P. andina fruits and to assess their antioxidant activity by means of chemical and cell-based assays. Methanol extracts from P. andina fruits were fractionated on a XAD7 resin and the main compounds were isolated by chromatographic means. Antioxidant activity was determined by means of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), ferric reducing power (FRAP), trolox equivalent antioxidant capacity (TEAC) and oxygen radical absorbance capacity (ORAC) assays. The cytoprotective activity of the extract against oxidative and dicarbonyl stress was evaluated in human gastric epithelial cells (AGS). The total intracellular antioxidant activity (TAA) of the extract was determined in AGS cells. The inhibition of meat lipoperoxidation was evaluated under simulated gastric digestion conditions. Rutin, caffeic acid β-glucoside and 20-hydroxyecdysone were identified as major components of the fruit extract. Additional compounds were identified by high-performance liquid chromatography diode-array detector mass spectrometry (HPLC-DAD-MSn) and/or co-injection with standards. Extracts showed dose-dependent cytoprotective effects against oxidative and dicarbonyl-induced damage in AGS cells. The TAA increased with the pre-incubation of AGS cells with the extract. This is the first report on the composition and biological activity of this Andean fruit. View Full-Text
Keywords: Prumnopitys andina; counter-current chromatography; 20-hydroxyecdysone; high-performance liquid chromatography; cell-based assays; thiobarbituric acid reactive species Prumnopitys andina; counter-current chromatography; 20-hydroxyecdysone; high-performance liquid chromatography; cell-based assays; thiobarbituric acid reactive species
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MDPI and ACS Style

Jiménez-Aspee, F.; Theoduloz, C.; Pormetter, L.; Mettke, J.; Ávila, F.; Schmeda-Hirschmann, G. Andean Prumnopitys Andina (Podocarpacae) Fruit Extracts: Characterization of Secondary Metabolites and Potential Cytoprotective Effect. Molecules 2019, 24, 4028. https://doi.org/10.3390/molecules24224028

AMA Style

Jiménez-Aspee F, Theoduloz C, Pormetter L, Mettke J, Ávila F, Schmeda-Hirschmann G. Andean Prumnopitys Andina (Podocarpacae) Fruit Extracts: Characterization of Secondary Metabolites and Potential Cytoprotective Effect. Molecules. 2019; 24(22):4028. https://doi.org/10.3390/molecules24224028

Chicago/Turabian Style

Jiménez-Aspee, Felipe; Theoduloz, Cristina; Pormetter, Lisa; Mettke, Judith; Ávila, Felipe; Schmeda-Hirschmann, Guillermo. 2019. "Andean Prumnopitys Andina (Podocarpacae) Fruit Extracts: Characterization of Secondary Metabolites and Potential Cytoprotective Effect" Molecules 24, no. 22: 4028. https://doi.org/10.3390/molecules24224028

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