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Open AccessArticle

Nematicidal Activities of Three Naphthoquinones against the Pine Wood Nematode, Bursaphelenchus xylophilus

Division of Forest Insects and Diseases Division, National Institute of Forest Science, Seoul 02455, Korea
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Molecules 2019, 24(20), 3634; https://doi.org/10.3390/molecules24203634
Received: 23 September 2019 / Revised: 6 October 2019 / Accepted: 7 October 2019 / Published: 9 October 2019
(This article belongs to the Section Natural Products Chemistry)
Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle, is a serious forest pest, causing enormous economic losses in pine trees in Korea, China, Japan, and countries in Western Europe. To prevent pine wilt disease (PWD), trunk injection with nematicide is performed in Korea. Although these nematicidal agents are quite efficient, the development of new nematicidal agents is needed to prevent pesticide resistance and reduce pest management costs. The aim of this study was to investigate nematicidal activities of pure naphthoquinones (NTQs)–1,4-NTQ, juglone, and plumbagin—against B. xylophilus via in vitro and semi-in vivo assays to identify new candidate agents for trunk injection. Estimated LC50 values (48 h exposure) were 100.0 ppm, 57.0 ppm, and 104.0 ppm for 1,4-NTQ, juglone, and plumbagin, respectively. In the semi-in vivo assay on pine bolt of the Japanese black pine, Pinus thunbergii, the population of inoculated B. xylophilus was significantly decreased at two weeks after treatment with juglone when compared with the effects of treatment with 1,4-NTQ and plumbagin. We also observed that naphthoquinones could generate reactive oxygen species, which presumably indicated that naphthoquinones caused significant oxidative stress in B. xylophilus. The findings of this study suggest the nematicidal potential of naphthoquinones and their possible use in further in vivo assays to test their nematicidal efficacy against B. xylophilus when injected through trunk injection. View Full-Text
Keywords: Bursaphelenchus xylophilus; naphthoquinone; reactive oxygen species Bursaphelenchus xylophilus; naphthoquinone; reactive oxygen species
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MDPI and ACS Style

Cha, D.J.; Kim, J.; Kim, D.S. Nematicidal Activities of Three Naphthoquinones against the Pine Wood Nematode, Bursaphelenchus xylophilus. Molecules 2019, 24, 3634.

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