Next Article in Journal
Phenomenological Inferences on the Kinetics of a Mechanically Activated Knoevenagel Condensation: Understanding the “Snowball” Kinetic Effect in Ball Milling
Next Article in Special Issue
Bimodular Antiparallel G-Quadruplex Nanoconstruct with Antiproliferative Activity
Previous Article in Journal
Detailed Analysis of the Influencing Parameters on the Self-Healing Behavior of Dynamic Urea-Crosslinked Poly(methacrylate)s
Previous Article in Special Issue
Energy Transfer as A Driving Force in Nucleic Acid–Protein Interactions
Open AccessReview

Inside the Black Box: What Makes SELEX Better?

Scientific-Manufacturing Complex Technological Centre, 1–7 Shokin Square, Zelenograd, Moscow 124498, Russia
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(19), 3598; https://doi.org/10.3390/molecules24193598
Received: 13 September 2019 / Revised: 4 October 2019 / Accepted: 4 October 2019 / Published: 7 October 2019
(This article belongs to the Special Issue Aptamers: Successes, Limitations and Future Directions)
Aptamers are small oligonucleotides that are capable of binding specifically to a target, with impressive potential for analysis, diagnostics, and therapeutics applications. Aptamers are isolated from large nucleic acid combinatorial libraries using an iterative selection process called SELEX (Systematic Evolution of Ligands by EXponential enrichment). Since being implemented 30 years ago, the SELEX protocol has undergone many modifications and improvements, but it remains a laborious, time-consuming, and costly method, and the results are not always successful. Each step in the aptamer selection protocol can influence its results. This review discusses key technical points of the SELEX procedure and their influence on the outcome of aptamer selection. View Full-Text
Keywords: aptamer; SELEX; nucleic acid library; PCR amplification; ssDNA regeneration; sequencing; next generation sequencing aptamer; SELEX; nucleic acid library; PCR amplification; ssDNA regeneration; sequencing; next generation sequencing
Show Figures

Figure 1

MDPI and ACS Style

Komarova, N.; Kuznetsov, A. Inside the Black Box: What Makes SELEX Better? Molecules 2019, 24, 3598.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop