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Open AccessArticle

Black Sorghum Phenolic Extract Regulates Expression of Genes Associated with Oxidative Stress and Inflammation in Human Endothelial Cells

1
School of Animal and Veterinary Sciences, Faculty of Science, Charles Sturt University, Wagga, NSW 2650, Australia
2
Australian Research Council (ARC) Industrial Transformation Training Centre for Functional Grains, Graham Centre for Agricultural Innovation, Charles Sturt University, Wagga, NSW 2650, Australia
3
School of Biomedical Sciences, Faculty of Science, Charles Sturt University, Wagga, NSW 2650, Australia
*
Author to whom correspondence should be addressed.
Academic Editors: Margarida Castell Escuer and Mariona Camps-Bossacoma
Molecules 2019, 24(18), 3321; https://doi.org/10.3390/molecules24183321
Received: 31 July 2019 / Revised: 10 September 2019 / Accepted: 10 September 2019 / Published: 12 September 2019
(This article belongs to the Special Issue Natural Polyphenols and Health)
Oxidative stress is one of the primary factors leading to endothelial dysfunction, a major underlying cause of vascular disorders. This study aims to understand the key signalling pathways regulated by sorghum (Shawaya short black 1 variety; characterised to be very high in its antioxidant activity) under oxidative stress in endothelial cells. Human umbilical vein endothelial cells (HUVECs) were pre-treated with non-cytotoxic concentrations of phenolic-rich black sorghum extract (BSE) prior to induction of oxidative stress using hydrogen peroxide (H2O2). Treatment with BSE upregulated the expression of heme oxygenase 1 (HO1) and endothelial nitric oxide synthase (eNOS) and downregulated the levels of NADPH oxidase 4 (NOX4). BSE treatment significantly reduced the expression of pro-inflammatory mediators such as monocyte chemoattractant protein 1 (MCP1) and intracellular adhesion molecule 1 (ICAM1). Results from this study suggest that phenolic-rich BSE may reduce oxidative stress by regulating pro- and antioxidant signalling pathways and the expression of inflammatory mediators linked to endothelial dysfunction under oxidative stress. View Full-Text
Keywords: black sorghum; genes; inflammation; oxidative stress; polyphenols black sorghum; genes; inflammation; oxidative stress; polyphenols
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MDPI and ACS Style

Francis, N.; Rao, S.; Blanchard, C.; Santhakumar, A. Black Sorghum Phenolic Extract Regulates Expression of Genes Associated with Oxidative Stress and Inflammation in Human Endothelial Cells. Molecules 2019, 24, 3321.

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