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Open AccessArticle

Analysis of Phenolic Composition of Byproducts (Seeds and Peels) of Avocado (Persea americana Mill.) Cultivated in Colombia

1
Departamento de Química, Universidad de Nariño, Pasto AA 1175, Nariño, Colombia
2
Departamento de Química, Universidad Nacional de Colombia, Bogotá DC AA 14490, Colombia
*
Author to whom correspondence should be addressed.
Academic Editor: Diego A. Moreno
Molecules 2019, 24(17), 3209; https://doi.org/10.3390/molecules24173209
Received: 3 July 2019 / Revised: 26 July 2019 / Accepted: 28 July 2019 / Published: 3 September 2019
(This article belongs to the Special Issue Bioactives and Functional Ingredients in Foods and Beverages)
The byproducts (seeds and peels) of an avocado cultivated in the south of Colombia were extracted with aqueous acetone and their antioxidant properties were measured with ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl) assays, and total polyphenol content was determined by Folin–Ciocalteu method. A bioguided fractionation was performed, first by SPE (solid phase extraction) on Amberlite XAD-7, and then by size exclusion chromatography on Sephadex LH-20. The polyphenolic-rich extracts and their fractions were analyzed by ultra-performance liquid chromatography–electrospray ionization–mass spectrometry (UPLC-ESI-MS/MS), finding the presence of organic acids, hydroxycinnamic acids, catechins, free and glycosylated flavonoids, and dimeric and trimeric procyanidins. Catechin, epicatechin, six quercetin derivatives, four dimeric procyanidins (three type B and one type A), and three trimeric procyanidins (two type B and one type A) were detected in the most active fractions of avocado peel and seeds. The most antioxidant fractions contain the higher molecular weight phenolic compounds (condensed tannins). View Full-Text
Keywords: polyphenols; Trolox equivalent antioxidant capacity (TEAC); DPPH; UPLC-ESI-MS/MS; bio-guided fractionation polyphenols; Trolox equivalent antioxidant capacity (TEAC); DPPH; UPLC-ESI-MS/MS; bio-guided fractionation
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MDPI and ACS Style

Rosero, J.C.; Cruz, S.; Osorio, C.; Hurtado, N. Analysis of Phenolic Composition of Byproducts (Seeds and Peels) of Avocado (Persea americana Mill.) Cultivated in Colombia. Molecules 2019, 24, 3209.

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