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Open AccessArticle

l-Buthionine Sulfoximine Detection and Quantification in Polyurea Dendrimer Nanoformulations

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CQFM-IN and IBB—Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal
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CEDOC, Chronic Diseases Research Centre, NOVA Medical School, Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, Campo dos Mártires da Pátria 130, 1169-056 Lisboa, Portugal
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Instituto Português de Oncologia de Lisboa Francisco Gentil (IPOLFG), Rua Prof. Lima Basto, 1099-023 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(17), 3111; https://doi.org/10.3390/molecules24173111
Received: 13 August 2019 / Revised: 19 August 2019 / Accepted: 20 August 2019 / Published: 27 August 2019
(This article belongs to the Section Analytical Chemistry)
l-Buthionine sulfoximine (l-BSO) is an adjuvant drug that is reported to increase the sensitivity of cancer cells to neoplastic agents. Dendrimers are exceptional drug delivery systems and l-BSO nanoformulations are envisaged as potential chemotherapeutics. The absorption of l-BSO at a low wavelength limits its detection by conventional analytical tools. A simple and sensitive method for l-BSO detection and quantification is now reported. In this study, l-BSO was encapsulated in a folate-targeted generation four polyurea dendrimer (PUREG4-FA2) and its release profile was followed for 24 h at pH 7.4 and 37 °C. The protocol uses in situ l-BSO derivatization, by the formation of a catechol-derived orto-quinone, followed by visible detection of the derivative at 503 nm. The structure of the studied l-BSO derivative was assessed by NMR spectroscopy. View Full-Text
Keywords: buthionine sulfoximine; polyurea dendrimers; catechol; nanoformulation; UV-Vis detection; derivatization buthionine sulfoximine; polyurea dendrimers; catechol; nanoformulation; UV-Vis detection; derivatization
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MDPI and ACS Style

Mota, P.; Pires, R.F.; Serpa, J.; Bonifácio, V.D.B. l-Buthionine Sulfoximine Detection and Quantification in Polyurea Dendrimer Nanoformulations. Molecules 2019, 24, 3111.

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