Next Article in Journal
Assessment of Antifouling Potential of Novel Transparent Sol Gel Coatings for Application in the Marine Environment
Previous Article in Journal
Molecular Imaging Probes Based on Matrix Metalloproteinase Inhibitors (MMPIs)
Open AccessArticle

Overcharging Effect in Electrospray Ionization Mass Spectra of Daunomycin-Tuftsin Bioconjugates

1
MTA-ELTE Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös Loránd University, Pázmány Péter sétány 1/A, 1117 Budapest, Hungary
2
Department of Organic Chemistry, Institute of Chemistry, Faculty of Science, ELTE Eötvös Loránd University, Pázmány Péter sétány 1/A, 1117 Budapest, Hungary
3
Department of Analytical Chemistry, Institute of Chemistry, Faculty of Science, ELTE Eötvös Loránd University, Pázmány Péter sétány 1/A, 1117 Budapest, Hungary
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(16), 2981; https://doi.org/10.3390/molecules24162981
Received: 22 July 2019 / Revised: 12 August 2019 / Accepted: 14 August 2019 / Published: 16 August 2019
Peptide-based small molecule drug conjugates for targeted tumor therapy are currently in the focus of intensive research. Anthracyclines, like daunomycin, are commonly used anticancer drug molecules and are also often applied in peptide-drug conjugates. However, lability of the O-glycosidic bond during electrospray ionization mass spectrometric analysis hinders the analytical characterization of the constructs. “Overprotonation” can occur if daunomycin is linked to positively charged peptide carriers, like tuftsin derivatives. In these molecules, the high number of positive charges enhances the in-source fragmentation significantly, leading to complex mass spectra composed of mainly fragment ions. Therefore, we investigated different novel tuftsin-daunomycin conjugates to find an appropriate condition for mass spectrometric detection. Our results showed that shifting the charge states to lower charges helped to keep ions intact. In this way, a clear spectrum could be obtained containing intact protonated molecules only. Shifting of the protonation states to lower charges could be achieved with the use of appropriate neutral volatile buffers and with tuning the ion source parameters. View Full-Text
Keywords: bioconjugates; peptides; anthracyclines; mass spectrometry; electrospray ionization; fragmentation bioconjugates; peptides; anthracyclines; mass spectrometry; electrospray ionization; fragmentation
Show Figures

Graphical abstract

MDPI and ACS Style

Pethő, L.; Mező, G.; Schlosser, G. Overcharging Effect in Electrospray Ionization Mass Spectra of Daunomycin-Tuftsin Bioconjugates. Molecules 2019, 24, 2981. https://doi.org/10.3390/molecules24162981

AMA Style

Pethő L, Mező G, Schlosser G. Overcharging Effect in Electrospray Ionization Mass Spectra of Daunomycin-Tuftsin Bioconjugates. Molecules. 2019; 24(16):2981. https://doi.org/10.3390/molecules24162981

Chicago/Turabian Style

Pethő, Lilla; Mező, Gábor; Schlosser, Gitta. 2019. "Overcharging Effect in Electrospray Ionization Mass Spectra of Daunomycin-Tuftsin Bioconjugates" Molecules 24, no. 16: 2981. https://doi.org/10.3390/molecules24162981

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop