Next Article in Journal
Exploration in the Mechanism of Action of Licorice by Network Pharmacology
Previous Article in Journal
Alpha-Hederin, the Active Saponin of Nigella sativa, as an Anticancer Agent Inducing Apoptosis in the SKOV-3 Cell Line
Previous Article in Special Issue
Stability Evaluation and Stabilization of a Gastrin-Releasing Peptide Receptor (GRPR) Targeting Imaging Pharmaceutical
Article Menu

Export Article

Open AccessArticle

Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues

1
National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov of the Ministry of Healthcare of Russian Federation, Moscow 117997, Russia
2
Department of Obstetrics, Gynecology, Perinatology and Reproductology, First Moscow State Medical University named after I.M. Sechenov, Moscow 119991, Russia
3
Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China University of Technology, Nanchang 330013, China
4
Moscow Institute of Physics and Technology, Moscow 141701, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Takashi Jin
Molecules 2019, 24(16), 2957; https://doi.org/10.3390/molecules24162957 (registering DOI)
Received: 29 May 2019 / Revised: 7 August 2019 / Accepted: 12 August 2019 / Published: 15 August 2019
(This article belongs to the Special Issue Development and Application of Molecular Imaging Probes/Techniques)
  |  
PDF [2734 KB, uploaded 21 August 2019]
  |     |  

Abstract

Real-time molecular navigation of tissue surgeries is an important goal at present. Combination of electrosurgical units and mass spectrometry (MS) to perform accurate molecular visualization of biological tissues has been pursued by many research groups. Determination of molecular tissue composition at a particular location by surgical smoke analysis is now of increasing interest for clinical use. However, molecular analysis of surgical smoke is commonly lacking molecular specificity and is associated with significant carbonization and chemical contamination, which are mainly related to the high temperature of smoke at which many molecules become unstable. Unlike traditional electrosurgical tools, low-temperature electrosurgical units allow tissue dissection without substantial heating. Here, we show that low-temperature electrosurgical units can be used for desorption of molecules from biological tissues without thermal degradation. The use of extractive electrospray ionization technique for the ionization of desorbed molecules allowed us to obtain mass spectra of healthy and pathological tissues with high degree of differentiation. Overall, the data indicate that the described approach has potential for intraoperative use. View Full-Text
Keywords: molecular imaging; mass spectrometry; EESI; breast cancer; intraoperative navigation molecular imaging; mass spectrometry; EESI; breast cancer; intraoperative navigation
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

Share & Cite This Article

MDPI and ACS Style

Sukhikh, G.; Chagovets, V.; Wang, X.; Rodionov, V.; Kometova, V.; Tokareva, A.; Kononikhin, A.; Starodubtseva, N.; Chingin, K.; Chen, H.; Frankevich, V. Combination of Low-Temperature Electrosurgical Unit and Extractive Electrospray Ionization Mass Spectrometry for Molecular Profiling and Classification of Tissues. Molecules 2019, 24, 2957.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top