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Chemical Profile and Antioxidant Activity of Zinnia elegans Jacq. Fractions

1
Department of Drug Analysis, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, Romania
2
Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation—State Research Institute, Czartoryskich 8, 24-100 Puławy, Poland
3
Department of Pharmaceutical Biochemistry and Clinical Laboratory, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, Romania
4
Department of Pharmacognosy, Faculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Street, 700115 Iasi, Romania
5
Center of Organic Chemistry “C.D. Nenitescu”, Romanian Academy, Spl. Independentei 202B, 060023 Bucharest, Romania
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Nazim Sekeroglu, Anake Kijjoa and Sevgi Gezici
Molecules 2019, 24(16), 2934; https://doi.org/10.3390/molecules24162934
Received: 30 July 2019 / Revised: 9 August 2019 / Accepted: 12 August 2019 / Published: 13 August 2019
(This article belongs to the Special Issue Selected Papers from the Joint Symposia of MESMAP-5 & ISPBS-5)
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Abstract

Zinnia elegans (syn. Zinnia violacea) is a common ornamental plant of the Asteraceae family, widely cultivated for the impressive range of flower colors and persistent bloom. Given its uncomplicated cultivation and high adaptability to harsh landscape conditions, we investigated the potential use of Z. elegans as a source of valuable secondary metabolites. Preliminary classification of compounds found in a methanolic extract obtained from inflorescences of Z. elegans cv. Caroussel was accomplished using HR LC-MS techniques. The extract was then subjected to solid-phase extraction and separation using Sephadex LH-20 column chromatography, which resulted in several fractions further investigated for their antioxidant properties through lipoxygenase inhibition and metal chelating activity assays. Moreover, following additional purification procedures, structures of some active ingredients were established by NMR spectroscopy. The investigated fractions contained polyphenolic compounds such as chlorogenic acids and apigenin, kaempferol, and quercetin glycosides. Antioxidant assays showed that certain fractions exhibit moderate 15-LOX inhibition (Fr 2, IC50 = 18.98 μg/mL) and metal chelation (e.g., Fr 1-2, EC50 = 0.714–1.037 mg/mL) activities as compared to positive controls (20.25 μg/mL for kaempferol and 0.068 mg/mL for EDTA, respectively). For Fr 2, the 15-LOX inhibition activity seems to be related to the abundance of kaempferol glycosides. The NMR analyses revealed the presence of a kaempferol 3-O-glycoside, and a guanidine alkaloid previously not described in this species. View Full-Text
Keywords: Zinnia elegans; Asteraceae; guanidine alkaloids; HR-QTOF/MS; lipoxygenase; metal chelation Zinnia elegans; Asteraceae; guanidine alkaloids; HR-QTOF/MS; lipoxygenase; metal chelation
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Burlec, A.F.; Pecio, Ł.; Mircea, C.; Cioancă, O.; Corciovă, A.; Nicolescu, A.; Oleszek, W.; Hăncianu, M. Chemical Profile and Antioxidant Activity of Zinnia elegans Jacq. Fractions. Molecules 2019, 24, 2934.

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