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Open AccessArticle

Extraction Optimization, Structural Characterization, and Antioxidant Activities of Polysaccharides from Cassia Seed (Cassia obtusifolia)

Institute of Food Processing and Safety, College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
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Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Molecules 2019, 24(15), 2817; https://doi.org/10.3390/molecules24152817
Received: 18 June 2019 / Revised: 30 July 2019 / Accepted: 31 July 2019 / Published: 2 August 2019
In order to explore Cassia seed polysaccharides (CSPs) as natural antioxidants for application in the functional-food industry, microwave-assisted extraction (MAE) was optimized for the extraction of CSPs by using a response surface methodology. Furthermore, the chemical structures and antioxidant activities of CSPs extracted by MAE and hot water extraction were investigated and compared. The maximum extraction yield of CSPs extracted by MAE (8.02 ± 0.19%) was obtained at the optimized extraction parameters as follows: microwave power (415 W), extraction time (7.0 min), and ratio of water to raw material (51 mL/g). Additionally, the contents of the uronic acids, molecular weight, ratio of constituent monosaccharides, intrinsic viscosities, and degrees of esterification of CSPs were significantly affected by the MAE method. Moreover, CSPs exhibited remarkable 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ABTS, 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl DPPH, nitric oxide, and hydroxyl radical scavenging activities as well as reducing power. The high antioxidant activities observed in CSPs extracted by MAE could be partially attributed to its low molecular weights and high content of unmethylated galacturonic acid. Results indicate that the MAE method could be an efficient technique for the extraction of CSPs with high antioxidant activity, and CSPs could be further explored as functional food ingredients. View Full-Text
Keywords: Cassia obtusifolia; polysaccharides; microwave-assisted extraction; chemical structure; antioxidant activity Cassia obtusifolia; polysaccharides; microwave-assisted extraction; chemical structure; antioxidant activity
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Wu, D.-T.; Liu, W.; Han, Q.-H.; Wang, P.; Xiang, X.-R.; Ding, Y.; Zhao, L.; Zhang, Q.; Li, S.-Q.; Qin, W. Extraction Optimization, Structural Characterization, and Antioxidant Activities of Polysaccharides from Cassia Seed (Cassia obtusifolia). Molecules 2019, 24, 2817.

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