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Article

Polysaccharides from Basidiocarps of Cultivating Mushroom Pleurotus ostreatus: Isolation and Structural Characterization

1
Department of Carbohydrates and Cereals, Faculty of Food and Biochemical Technology, University of Chemistry and Technology in Prague, Technická 5, 166 28 Praha 6 Dejvice, Czech Republic
2
Department of Gardening, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Praha 6 Suchdol, Czech Republic
3
Department of Crop Production, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Praha 6 Suchdol, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Luca Vannucci
Molecules 2019, 24(15), 2740; https://doi.org/10.3390/molecules24152740
Received: 30 June 2019 / Revised: 22 July 2019 / Accepted: 27 July 2019 / Published: 28 July 2019
(This article belongs to the Special Issue Effects of Beta Glucan As Natural Immunomodulator)
Oyster mushrooms are an interesting source of biologically active glucans and other polysaccharides. This work is devoted to the isolation and structural characterization of polysaccharides from basidiocarps of the cultivated oyster mushroom, Pleurotus ostreatus. Five polysaccharidic fractions were obtained by subsequent extraction with cold water, hot water and two subsequent extractions with 1 m sodium hydroxide. Branched partially methoxylated mannogalactan and slightly branched (1→6)-β-d-glucan predominated in cold- and hot-water-soluble fractions, respectively. Alternatively, these polysaccharides were obtained by only hot water extraction and subsequent two-stage chromatographic separation. The alkali-soluble parts originating from the first alkali extraction were then fractionated by dissolution in dimethyl sulfoxide (DMSO). The polysaccharide insoluble in DMSO was identified as linear (1→3)-α-d-glucan, while branched (1→3)(1→6)-β-d-glucans were found to be soluble in DMSO. The second alkaline extract contained the mentioned branched β-d-glucan together with some proteins. Finally, the alkali insoluble part was a cell wall complex of chitin and β-d-glucans. View Full-Text
Keywords: oyster mushrooms; basidiocarps; polysaccharides; fractionation; mannogalactan; glucans oyster mushrooms; basidiocarps; polysaccharides; fractionation; mannogalactan; glucans
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MDPI and ACS Style

Baeva, E.; Bleha, R.; Lavrova, E.; Sushytskyi, L.; Čopíková, J.; Jablonsky, I.; Klouček, P.; Synytsya, A. Polysaccharides from Basidiocarps of Cultivating Mushroom Pleurotus ostreatus: Isolation and Structural Characterization. Molecules 2019, 24, 2740. https://doi.org/10.3390/molecules24152740

AMA Style

Baeva E, Bleha R, Lavrova E, Sushytskyi L, Čopíková J, Jablonsky I, Klouček P, Synytsya A. Polysaccharides from Basidiocarps of Cultivating Mushroom Pleurotus ostreatus: Isolation and Structural Characterization. Molecules. 2019; 24(15):2740. https://doi.org/10.3390/molecules24152740

Chicago/Turabian Style

Baeva, Ekaterina, Roman Bleha, Ekaterina Lavrova, Leonid Sushytskyi, Jana Čopíková, Ivan Jablonsky, Pavel Klouček, and Andriy Synytsya. 2019. "Polysaccharides from Basidiocarps of Cultivating Mushroom Pleurotus ostreatus: Isolation and Structural Characterization" Molecules 24, no. 15: 2740. https://doi.org/10.3390/molecules24152740

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