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Analysis of the Overlapped Electrochemical Signals of Hydrochlorothiazide and Pyridoxine on the Ethylenediamine-Modified Glassy Carbon Electrode by Use of Chemometrics Methods

1
Port of Caofeidian Group Co., Ltd., Tangshan 063200, China
2
College of Medicine, Nanchang University, Nanchang 330031, China
3
Department of Chemistry, Nanchang University, Nanchang 330031, China
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Academic Editors: Clinio Locatelli, Marcello Locatelli and Dora Melucci
Molecules 2019, 24(14), 2536; https://doi.org/10.3390/molecules24142536
Received: 1 June 2019 / Revised: 3 July 2019 / Accepted: 9 July 2019 / Published: 11 July 2019
(This article belongs to the Special Issue Analytical Methods for Toxics Determination)
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Abstract

In this work, the electrochemical behavior of hydrochlorothiazide and pyridoxine on the ethylenediamine-modified glassy carbon electrode were investigated by differential pulse voltammetry. In pH 3.4 Britton-Robinson (B-R) buffer solution, both hydrochlorothiazide and pyridoxine had a pair of sensitive irreversible oxidation peaks, that overlapped in the 1.10 V to 1.20 V potential range. Under the optimum experimental conditions, the peak current was linearly related to hydrochlorothiazide and pyridoxine in the concentration range of 0.10–2.0 μg/mL and 0.02–0.40 μg/mL, respectively. Chemometrics methods, including classical least squares (CLS), principal component regression (PCR) and partial least squares (PLS), were introduced to resolve the overlapped signals and determine the two components in mixtures, which avoided the troublesome steps of separation and purification. Finally, the simultaneous determination of the two components in commercial pharmaceuticals was performed with satisfactory results. View Full-Text
Keywords: differential pulse voltammetry; hydrochlorothiazide; pyridoxine; chemometrics differential pulse voltammetry; hydrochlorothiazide; pyridoxine; chemometrics
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Zhang, Y.; Zhou, Y.; Chen, S.; You, Y.; Qiu, P.; Ni, Y. Analysis of the Overlapped Electrochemical Signals of Hydrochlorothiazide and Pyridoxine on the Ethylenediamine-Modified Glassy Carbon Electrode by Use of Chemometrics Methods. Molecules 2019, 24, 2536.

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