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An LC-MS/MS Method to Measure S-Methyl-l-Cysteine and S-Methyl-l-Cysteine Sulfoxide in Human Specimens Using Isotope Labelled Internal Standards

1
Quadram Institute Bioscience, Norwich NR4 7UQ, UK
2
Department of Urology, Norfolk and Norwich University Hospitals NHS Foundation Trust, Norwich NR4 7UY, UK
*
Author to whom correspondence should be addressed.
These authors contributed equally to the work.
Molecules 2019, 24(13), 2427; https://doi.org/10.3390/molecules24132427
Received: 1 May 2019 / Revised: 28 June 2019 / Accepted: 29 June 2019 / Published: 2 July 2019
(This article belongs to the Special Issue Biological Sample Analysis by Liquid Chromatography)
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Abstract

This is the first report describing an analytical method for quantitative analysis of two naturally occurring sulphur compounds, S-methyl-l-cysteine (SMC) and S-methyl-l-cysteine sulfoxide (SMCSO), in human body fluids using isotope-labelled internal standards and liquid chromatography-mass spectrometry (LC-MS)/MS techniques. This method was validated according to the guideline of the Royal Society of Chemistry Analytical Methods Committee. It offers significant advantages including simple and fast preparation of human biological samples. The limits of detection of SMC were 0.08 µM for urine and 0.04 µM for plasma. The limits of detection of SMCSO were 0.03 µM for urine and 0.02 µM for plasma. The calibration curves of all matrices showed linearity with correlation coefficients r2 > 0.9987. The intra and inter day precisions in three levels of known concentrations were >10% and >20%, respectively. The quantification accuracy was 98.28 ± 5.66%. The proposed method would be beneficial for the rapid and accurate determination of the SMC and SMCSO in human plasma and urine samples using by isotope labelled internal standards. View Full-Text
Keywords: S-methyl-l-cysteine; S-methyl-l-cysteine sulfoxide; sulphur compounds; liquid chromatography tandem mass spectrometry; body fluids S-methyl-l-cysteine; S-methyl-l-cysteine sulfoxide; sulphur compounds; liquid chromatography tandem mass spectrometry; body fluids
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Sivapalan, T.; Melchini, A.; Coode-Bate, J.; Needs, P.W.; Mithen, R.F.; Saha, S. An LC-MS/MS Method to Measure S-Methyl-l-Cysteine and S-Methyl-l-Cysteine Sulfoxide in Human Specimens Using Isotope Labelled Internal Standards. Molecules 2019, 24, 2427.

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