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Use of Indocyanine Green (ICG), a Medical Near Infrared Dye, for Enhanced Fluorescent Imaging—Comparison of Organic Anion Transporting Polypeptide 1B3 (OATP1B3) and Sodium-Taurocholate Cotransporting Polypeptide (NTCP) Reporter Genes

1
Institute of Biomedical Engineering, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan
2
Department of Medical Imaging, Taipei TzuChi General Hospital, Buddhist Tzu-Chi Medical Foundation, No.289, Jianguo Rd., Xindian Dist., New Taipei city 23142, Taiwan
3
School of Medicine, Tzu Chi University, No. 701, Sec. 3, Zhongyang Rd. Hualien 97004, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Maged Henary
Molecules 2019, 24(12), 2295; https://doi.org/10.3390/molecules24122295
Received: 27 May 2019 / Revised: 11 June 2019 / Accepted: 20 June 2019 / Published: 21 June 2019
Molecular and cellular imaging in living organisms have ushered in an era of comprehensive understanding of intracellular and intercellular events. Currently, more efforts have been focused on the infrared fluorescent dyes that facilitate deeper tissue visualization. Both sodium taurocholate cotransporting polypeptide (NTCP) and organic-anion-transporting polypeptide 1B3 (OATP1B3) are capable of carrying indocyanine green (ICG) into the cytoplasm. We compared the feasibility of NTCP and OATP1B3 as reporter genes in combination with ICG. NTCP and OATP1B3 were transduced into HT-29 cells. Genetically modified HT-29 cells were inoculated into nude mice. ICG was administered in vitro and in vivo and the signals were observed under confocal microscopy, flow cytometry, multimode microplate reader, and an in vivo imaging system. Both NTCP- and OATP1B3-expressing cells and xenografts had higher ICG intensities. The OATP1B3-expressing xenograft has a higher ICG uptake than the NTCP-expressing xenograft. NTCP or OATP1B3 combined with ICG could serve as a noninvasive imaging modality for molecular and cellular imaging. OATP1B3 outperforms NTCP in terms of in vivo imaging. View Full-Text
Keywords: sodium taurocholate cotransporting polypeptide (NTCP); organic-anion-transporting polypeptide 1B3 (OATP1B3); indocyanine green (ICG); in vivo imaging system (IVIS) sodium taurocholate cotransporting polypeptide (NTCP); organic-anion-transporting polypeptide 1B3 (OATP1B3); indocyanine green (ICG); in vivo imaging system (IVIS)
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Wu, M.-R.; Huang, Y.-Y.; Hsiao, J.-K. Use of Indocyanine Green (ICG), a Medical Near Infrared Dye, for Enhanced Fluorescent Imaging—Comparison of Organic Anion Transporting Polypeptide 1B3 (OATP1B3) and Sodium-Taurocholate Cotransporting Polypeptide (NTCP) Reporter Genes. Molecules 2019, 24, 2295.

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