Next Article in Journal
Genome-Guided Discovery of Pretilactam from Actinosynnema pretiosum ATCC 31565
Previous Article in Journal
Ratiometric Fluorescent Nanoprobe for Highly Sensitive Determination of Mercury Ions
Article Menu
Issue 12 (June-2) cover image

Export Article

Open AccessArticle

The Antibiofilm Effect of a Medical Device Containing TIAB on Microorganisms Associated with Surgical Site Infection

1
Department of Medicine and Aging Science, “G. d′Annunzio” of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy
2
Center of Excellence on Aging and Translational Medicine (CeSI-MeT), Via Luigi Polacchi 11, 66100 Chieti, Italy
3
Department of Medical, Oral, and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy
4
Department of Neuroscience Imaging and Clinical Sciences, “G. d′Annunzio” University Chieti-Pescara, Via Luigi Polacchi 11, 66100 Chieti, Italy
5
Department of Pharmacy, “G. d’Annunzio” University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy
6
Department of Biomedical, Surgical and Dental Sciences, University of Milan, Via della Commenda 10, 20122 Milan, Italy
7
Department of Urology, University of Milan, ASST Santi Paolo e Carlo, Via Antonio di Rudinì 8, 20142 Milan, Italy
*
Author to whom correspondence should be addressed.
This authors contributed equally to this work.
Academic Editors: Manuel Simões, Sérgio F. Sousa and Efstathios Giaouris
Molecules 2019, 24(12), 2280; https://doi.org/10.3390/molecules24122280
Received: 14 May 2019 / Revised: 10 June 2019 / Accepted: 18 June 2019 / Published: 19 June 2019
(This article belongs to the Special Issue Biofilm Control)
  |  
PDF [8756 KB, uploaded 19 June 2019]
  |  

Abstract

Surgical site infections (SSIs) represent the most common nosocomial infections, and surgical sutures are optimal surfaces for bacterial adhesion and biofilm formation. Staphylococcus spp., Enterococcus spp., and Escherichia coli are the most commonly isolated microorganisms. The aim of this research was to evaluate the antibiofilm activity of a medical device (MD) containing TIAB, which is a silver-nanotech patented product. The antibacterial effect was evaluated against Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, and E. coli ATCC 25922 by assessing the minimum inhibitory concentration (MIC) by the Alamar Blue® (AB) assay. The antibiofilm effect was determined by evaluation of the minimum biofilm inhibitory concentration (MBIC) and colony-forming unit (CFU) count. Subsequently, the MD was applied on sutures exposed to the bacterial species. The antimicrobial and antibiofilm effects were evaluated by the agar diffusion test method, confocal laser scanning microscopy (CLSM), and scanning electron microscopy (SEM). The MIC was determined for S. aureus and E. faecalis at 2 mg/mL, while the MBIC was 1.5 mg/mL for S. aureus and 1 mg/mL for E. faecalis. The formation of an inhibition zone around three different treated sutures confirmed the antimicrobial activity, while the SEM and CLSM analysis performed on the MD-treated sutures underlined the presence of a few adhesive cells, which were for the most part dead. The MD showed antimicrobial and antibiofilm activities versus S. aureus and E. faecalis, but a lower efficacy against E. coli. Surgical sutures coated with the MD have the potential to reduce SSIs as well as the risk of biofilm formation post-surgery. View Full-Text
Keywords: Staphylococcus aureus; Enterococcus faecalis; antimicrobial activity; antibiofilm activity; surgical site infection; TIAB Staphylococcus aureus; Enterococcus faecalis; antimicrobial activity; antibiofilm activity; surgical site infection; TIAB
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Puca, V.; Traini, T.; Guarnieri, S.; Carradori, S.; Sisto, F.; Macchione, N.; Muraro, R.; Mincione, G.; Grande, R. The Antibiofilm Effect of a Medical Device Containing TIAB on Microorganisms Associated with Surgical Site Infection. Molecules 2019, 24, 2280.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top