Next Article in Journal
Design, Synthesis and Cancer Cell Growth Inhibition Evaluation of New Aminoquinone Hybrid Molecules
Previous Article in Journal
Dioscin Inhibits the Invasion and Migration of Hepatocellular Carcinoma HepG2 Cells by Reversing TGF-β1-Induced Epithelial-Mesenchymal Transition
Open AccessArticle

Identification of a Heat-Inducible Element of Cysteine Desulfurase Gene Promoter in Lentinula edodes

1
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
2
Department of Nutrition, Food Science and Packaging, California State University, San Jose, CA 95192, USA
3
Institute of Applied Mycology, Plant Science and Technology College, Huazhong Agricultural University, Wuhan 430070, China
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(12), 2223; https://doi.org/10.3390/molecules24122223
Received: 25 April 2019 / Revised: 29 May 2019 / Accepted: 13 June 2019 / Published: 14 June 2019
(This article belongs to the Section Chemical Biology)
Volatile organosulfur compounds are the main components that contribute to the unique aroma of dried Lentinula edodes. They are mainly generated during the hot-air drying process, and cysteine desulfurase is the key enzyme in this process. Temperature may be an essential factor of volatile organosulfur compound production by influencing the expression of the cysteine desulfurase gene. In this study, the promoter sequence of the cysteine desulfurase gene (pCS) was cloned and analyzed using bioinformatics tools. A series of 5′deletion fragments and site-directed mutations of pCS were constructed to identify the element that responds to heat stress. Six heat shock transcription factor (HSTF) binding sites were predicted by SCPD (The Promoter Database of Saccharomyces cerevisiae) and three of the binding sites were predicted by Yeastract (Yeast Search for Transcriptional Regulators and Consensus Tracking) in pCS. The results indicated that pCS was able to drive the expression of the EGFP (Enhanced Green Fluorescent Protein) gene in L. edodes. Moreover, the fluorescence intensity increased after heat stress. The changes in fluorescence intensity of different 5′deletion fragments showed that the heat response region was located between −500 bp and −400 bp in pCS. The site-directed mutation analysis further showed that the heat-inducible element was between −490 bp and −500 bp (TTTCTAGAAT) in pCS. Our results provide molecular insight for studying the formation of volatile organosulfur compounds in dried L. edodes. View Full-Text
Keywords: Lentinula edodes; gene promoter; volatile organosulfur compounds; heat-inducible element Lentinula edodes; gene promoter; volatile organosulfur compounds; heat-inducible element
Show Figures

Figure 1

MDPI and ACS Style

Huang, Z.; Lei, X.; Feng, X.; Gao, S.; Wang, G.; Bian, Y.; Huang, W.; Liu, Y. Identification of a Heat-Inducible Element of Cysteine Desulfurase Gene Promoter in Lentinula edodes. Molecules 2019, 24, 2223.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop