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Open AccessArticle

Mass Spectrometry-Based Untargeted Metabolomics and α-Glucosidase Inhibitory Activity of Lingzhi (Ganoderma lingzhi) During the Developmental Stages

1
Division of Systematic Forest and Forest Products Sciences, Department of Agro-Environmental Sciences, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan
2
Faculty of Health and Sciences, Muhammadiyah University of Sumatera Barat, Bukittinggi 26181, Indonesia
3
Miyazaki Prefectural Wood Utilization Research Center, Miyazaki 885-0037, Japan
4
Fukuoka Prefecture Forest Research & Extension Center, Fukuoka 818-8549, Japan
*
Author to whom correspondence should be addressed.
(S.K. formerly worked in Fukuoka Prefecture Forest Research & Extension Center).
Academic Editor: Thomas Letzel
Molecules 2019, 24(11), 2044; https://doi.org/10.3390/molecules24112044
Received: 26 April 2019 / Revised: 23 May 2019 / Accepted: 27 May 2019 / Published: 29 May 2019
(This article belongs to the Special Issue (Mass Spectrometric) Non Target Screening–Techniques and Strategies)
Lingzhi is a Ganoderma mushroom species which has a wide range of bioactivities. Analysis of the changes in metabolites during the developmental stages of lingzhi is important to understand the underlying mechanism of its biosynthesis, as well as its bioactivity. It may also provide valuable information for the cultivation efficiency of lingzhi. In this study, mass spectrometry based untargeted metabolomics was carried out to analyze the alteration of metabolites during developmental stages of lingzhi. Eight developmental stages were categorized on the basis of morphological changes; starting from mycelium stage to post-mature stage. GC/MS and LC/MS analyses along with multivariate analysis of lingzhi developmental stages were performed. Amino acids, organic acids, sugars, polyols, fatty acids, fatty alcohols, and some small polar metabolites were extracted as marker metabolites from GC/MS analysis, while, lanostane-type triterpenoids were observed in LC/MS analysis of lingzhi. The marker metabolites from untargeted analysis of lingzhi developmental stages were correlated with the α-glucosidase inhibitory activity. Two metabolites, compounds 34 and 35, were identified as potential contributors of the α-glucosidase inhibitory activity. The current result shows that some metabolites are involved in the developmental process and α-glucosidase inhibitory activity of lingzhi. View Full-Text
Keywords: Ganoderma lingzhi; developmental stages; untargeted metabolomics; GC/MS; LC/IT-TOF-MS; α-glucosidase inhibitory activity Ganoderma lingzhi; developmental stages; untargeted metabolomics; GC/MS; LC/IT-TOF-MS; α-glucosidase inhibitory activity
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MDPI and ACS Style

Satria, D.; Tamrakar, S.; Suhara, H.; Kaneko, S.; Shimizu, K. Mass Spectrometry-Based Untargeted Metabolomics and α-Glucosidase Inhibitory Activity of Lingzhi (Ganoderma lingzhi) During the Developmental Stages. Molecules 2019, 24, 2044.

AMA Style

Satria D, Tamrakar S, Suhara H, Kaneko S, Shimizu K. Mass Spectrometry-Based Untargeted Metabolomics and α-Glucosidase Inhibitory Activity of Lingzhi (Ganoderma lingzhi) During the Developmental Stages. Molecules. 2019; 24(11):2044.

Chicago/Turabian Style

Satria, Dedi; Tamrakar, Sonam; Suhara, Hiroto; Kaneko, Shuhei; Shimizu, Kuniyoshi. 2019. "Mass Spectrometry-Based Untargeted Metabolomics and α-Glucosidase Inhibitory Activity of Lingzhi (Ganoderma lingzhi) During the Developmental Stages" Molecules 24, no. 11: 2044.

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