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Characterization of Cultivar Differences of Blueberry Wines Using GC-QTOF-MS and Metabolic Profiling Methods

1,2,*, 1, 1 and 1,2
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, Wuhan 430070, China
Author to whom correspondence should be addressed.
Molecules 2018, 23(9), 2376;
Received: 3 September 2018 / Revised: 14 September 2018 / Accepted: 15 September 2018 / Published: 17 September 2018
(This article belongs to the Special Issue Technology for Natural Products Research)
PDF [1665 KB, uploaded 17 September 2018]


A non-targeted volatile metabolomic approach based on the gas chromatography-quadrupole time of fight-mass spectrometry (GC-QTOF-MS) coupled with two different sample extraction techniques (solid phase extraction and solid phase microextraction) was developed. Combined mass spectra of blueberry wine samples, which originated from two different cultivars, were subjected to orthogonal partial least squares-discriminant analysis (OPLS-DA). Principal component analysis (PCA) reveals an excellent separation and OPLS-DA highlight metabolic features responsible for the separation. Metabolic features responsible for the observed separation were tentatively assigned to phenylethyl alcohol, cinnamyl alcohol, benzenepropanol, 3-hydroxy-benzenethanol, methyl eugenol, methyl isoeugenol, (E)-asarone, (Z)-asarone, and terpenes. Several of the selected markers enabled a distinction in secondary metabolism to be drawn between two blueberry cultivars. It highlights the metabolomic approaches to find out the influence of blueberry cultivar on a volatile composition in a complex blueberry wine matrix. The distinction in secondary metabolism indicated a possible O-methyltransferases activity difference among the two cultivars. View Full-Text
Keywords: blueberry wine; volatile composition; multivariate analysis; GC-QTOF-MS analysis blueberry wine; volatile composition; multivariate analysis; GC-QTOF-MS analysis

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Yuan, F.; Cheng, K.; Gao, J.; Pan, S. Characterization of Cultivar Differences of Blueberry Wines Using GC-QTOF-MS and Metabolic Profiling Methods. Molecules 2018, 23, 2376.

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