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Molecules 2018, 23(7), 1736; https://doi.org/10.3390/molecules23071736

Quick Multi-Class Determination of Residues of Antimicrobial Veterinary Drugs in Animal Muscle by LC-MS/MS

1
National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
2
Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics, Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China
The authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 4 June 2018 / Revised: 10 July 2018 / Accepted: 11 July 2018 / Published: 16 July 2018
(This article belongs to the Special Issue Trends in Veterinary Drug Analysis: Multiresidue and Omic Approaches)
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Abstract

On the basis of the highly sensitive and selective liquid chromatography-tandem mass spectrometry technique, a generic extraction solvent and a sample dilution method was developed for the residue analysis of different polar veterinary drugs known as fluoroquinolones, sulfonamides, macrolides, and tiamulin in chicken muscle. The results showed that the matrix-matched calibration curves of all 10 compounds were in an effective linear relationship (r2 ≥ 0.997) in the range of 0.2–100 μg L−1. At three spiking levels of 2 (5), 50, and 100 μg kg−1, average recoveries of analytes were between 67.1% and 96.6% with relative standard deviations of intra-day and inter-day below 20%. The limits of detection and limits of quantification of the method were in the range of 0.3–2.0 μg kg−1 and 2.0–5.0 μg kg−1, respectively, which were significantly lower than their maximum residue limits. In addition, the intensity of the target analytes and its corresponding matrix effects were obviously related to the sample dilution times (matrix concentration). There were no significant differences (p > 0.05) in the average content of almost any of the analytes in medicated chickens between this method and the method in the literature for determining analytes. Lastly, the proposed method was successfully applied for the simultaneous analysis of 10 common veterinary drugs in food animal muscle tissues. View Full-Text
Keywords: veterinary drugs; generic extraction solvent; matrix effects; liquid chromatography-tandem mass spectrometry; dilution strategy; animal muscle veterinary drugs; generic extraction solvent; matrix effects; liquid chromatography-tandem mass spectrometry; dilution strategy; animal muscle
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Zhang, M.; Li, E.; Su, Y.; Zhang, Y.; Xie, J.; He, L. Quick Multi-Class Determination of Residues of Antimicrobial Veterinary Drugs in Animal Muscle by LC-MS/MS. Molecules 2018, 23, 1736.

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