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Open AccessArticle

A Modified GC-MS Analytical Procedure for Separation and Detection of Multiple Classes of Carbohydrates

Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, Heilongjiang, China
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Molecules 2018, 23(6), 1284; https://doi.org/10.3390/molecules23061284
Received: 8 May 2018 / Revised: 21 May 2018 / Accepted: 21 May 2018 / Published: 27 May 2018
(This article belongs to the Section Analytical Chemistry)
A modified GC-MS analytical procedure based on trimethylsilyl-dithioacetal (TMSD) derivatization has been established for a simultaneous determination of thirteen carbohydrates. Different from previous approaches, the current GC-MS method was featured by a powerful practicability for simultaneous detection of aldoses, uronic acids, ketoses, and amino sugars; simplifying GC-MS chromatograms and producing a single peak for each derivatized sugar, as well as high resolution, sensitivity, and repeatability. An additional liquid-liquid extraction from derivatization mixtures was performed not only to increase the detection sensitivity of amino sugars but also to decrease the by-products of derivatization. Contrarily, three amino sugars were detected at a very low intensity or not detected at all. The effect of time on monosaccharide- mercaptalated reaction was systematically investigated. The effect of trimethylsilylation on the formation of TMSD was also optimized. The established GC-MS based on TMSD derivatization was suitable for complex carbohydrate analysis and has been successfully applied for the detection of free carbohydrates in water extracts of Anemarrhena asphodeloides roots and determination of monosaccharides in Glossy ganoderma polysaccharides. View Full-Text
Keywords: GC-MS; trimethylsilyl-dithioacetal; ketose; amino sugar; aldoses; uronic acids GC-MS; trimethylsilyl-dithioacetal; ketose; amino sugar; aldoses; uronic acids
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Xia, Y.-G.; Sun, H.-M.; Wang, T.-L.; Liang, J.; Yang, B.-Y.; Kuang, H.-X. A Modified GC-MS Analytical Procedure for Separation and Detection of Multiple Classes of Carbohydrates. Molecules 2018, 23, 1284.

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