Next Article in Journal
A 3D-QSAR Study on the Antitrypanosomal and Cytotoxic Activities of Steroid Alkaloids by Comparative Molecular Field Analysis
Next Article in Special Issue
Ultrasonically Modified Amended-Cloud Point Extraction for Simultaneous Pre-Concentration of Neonicotinoid Insecticide Residues
Previous Article in Journal
Semisynthesis, Characterization and Evaluation of New Adenosine Derivatives as Antiproliferative Agents
Previous Article in Special Issue
Greening Reversed-Phase Liquid Chromatography Methods Using Alternative Solvents for Pharmaceutical Analysis
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle
Molecules 2018, 23(5), 1112; https://doi.org/10.3390/molecules23051112

Determination of Biogenic Amines in Seawater Using Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection

1
School of Chemical Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia
2
Chemistry Department, Faculty of Science, University of Khartoum, Khartoum 11115, Sudan
3
Department of Chemistry, Faculty of Science, University of Jordan, Amman 11942, Jordan
4
Australian Centre for Research on Separation Science (ACROSS), School of Physical Sciences-Chemistry, University of Tasmania, Hobart 7001, Australia
5
Fundamental & Applied Sciences Department and Institute for Sustainable Living, Universiti Teknologi PETRONAS, Seri Iskandar 32610, Perak, Malaysia
*
Authors to whom correspondence should be addressed.
Received: 17 April 2018 / Revised: 30 April 2018 / Accepted: 3 May 2018 / Published: 8 May 2018
(This article belongs to the Special Issue Green Analytical Chemistry)
Full-Text   |   PDF [1785 KB, uploaded 8 May 2018]   |  

Abstract

A rapid and green analytical method based on capillary electrophoresis with capacitively coupled contactless conductivity detection (C4D) for the determination of eight environmental pollutants, the biogenic amines (putrescine, cadaverine, spermidine, spermine, tyramine, 2-phenylamine, histamine and tryptamine), is described. The separation was achieved under normal polarity mode at 24 °C and 25 kV with a hydrodynamic injection (50 mbar for 5 s) and using a bare fused-silica capillary (95 cm length × 50 µm i.d.) (detection length of 10.5 cm from the outlet end of the capillary). The optimized background electrolyte consisted of 400 mM malic acid. C4D parameters were set at a fixed amplitude (50 V) and frequency (600 kHz). Under the optimum conditions, the method exhibited good linearity over the range of 1.0–100 µg mL−1 (R2 ≥ 0.981). The limits of detection based on signal to noise (S/N) ratios of 3 and 10 were ≤0.029 µg mL−1. The method was used for the determination of seawater samples that were spiked with biogenic amines. Good recoveries (77–93%) were found. View Full-Text
Keywords: capillary electrophoresis; capacitively coupled contactless conductivity detector; biogenic amines; seawater capillary electrophoresis; capacitively coupled contactless conductivity detector; biogenic amines; seawater
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Gubartallah, E.A.; Makahleh, A.; Quirino, J.P.; Saad, B. Determination of Biogenic Amines in Seawater Using Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection. Molecules 2018, 23, 1112.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top