Kaempferol Induces G2/M Cell Cycle Arrest via Checkpoint Kinase 2 and Promotes Apoptosis via Death Receptors in Human Ovarian Carcinoma A2780/CP70 Cells

Gao, Ying; Yin, Junfeng; Rankin, Gary O.; Chen, Yi Charlie

doi:10.3390/molecules23051095

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Kaempferol Induces G2/M Cell Cycle Arrest via Checkpoint Kinase 2 and Promotes Apoptosis via Death Receptors in Human Ovarian Carcinoma A2780/CP70 Cells

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Tea Research Institute Chinese Academy of Agricultural Sciences, Ministry of Agriculture, Hangzhou 310008, China

Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25755, USA

College of Science, Technology and Mathematics, Alderson Broaddus University, Philippi, WV 26416, USA

Author to whom correspondence should be addressed.

Molecules 2018, 23(5), 1095; https://doi.org/10.3390/molecules23051095

Received: 15 April 2018 / Revised: 3 May 2018 / Accepted: 4 May 2018 / Published: 5 May 2018

(This article belongs to the Section Natural Products Chemistry)

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Abstract

Kaempferol is a widely distributed dietary flavonoid. Epidemiological studies have demonstrated kaempferol consumption lowers the risk of ovarian cancer. Our previous research proved that kaempferol suppresses human ovarian cancer cells by inhibiting tumor angiogenesis. However, the effects of kaempferol on the cell cycle and extrinsic apoptosis of ovarian cancer cells have not yet been studied. In the present study, we demonstrated that kaempferol induced G2/M cell cycle arrest via the Chk2/Cdc25C/Cdc2 pathway and Chk2/p21/Cdc2 pathway in human ovarian cancer A2780/CP70 cells. Chk2 was not responsible for kaempferol-induced apoptosis and up-regulation of p53. Kaempferol stimulated extrinsic apoptosis via death receptors/FADD/Caspase-8 pathway. Our study suggested that Chk2 and death receptors played important roles in the anticancer activity of kaempferol in A2780/CP70 cells. These findings provide more evidence of the anti-ovarian cancer properties of kaempferol and suggest that kaempferol could be a potential candidate for ovarian cancer adjuvant therapy. View Full-Text

Keywords: kaempferol; cell cycle arrest; Chk2; apoptosis; DR5; Fas

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Gao, Y.; Yin, J.; Rankin, G.O.; Chen, Y.C. Kaempferol Induces G2/M Cell Cycle Arrest via Checkpoint Kinase 2 and Promotes Apoptosis via Death Receptors in Human Ovarian Carcinoma A2780/CP70 Cells. Molecules 2018, 23, 1095. https://doi.org/10.3390/molecules23051095

AMA Style

Gao Y, Yin J, Rankin GO, Chen YC. Kaempferol Induces G2/M Cell Cycle Arrest via Checkpoint Kinase 2 and Promotes Apoptosis via Death Receptors in Human Ovarian Carcinoma A2780/CP70 Cells. Molecules. 2018; 23(5):1095. https://doi.org/10.3390/molecules23051095

Chicago/Turabian Style

Gao, Ying, Junfeng Yin, Gary O. Rankin, and Yi C. Chen. 2018. "Kaempferol Induces G2/M Cell Cycle Arrest via Checkpoint Kinase 2 and Promotes Apoptosis via Death Receptors in Human Ovarian Carcinoma A2780/CP70 Cells" Molecules 23, no. 5: 1095. https://doi.org/10.3390/molecules23051095

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Kaempferol Induces G2/M Cell Cycle Arrest via Checkpoint Kinase 2 and Promotes Apoptosis via Death Receptors in Human Ovarian Carcinoma A2780/CP70 Cells

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