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Molecules 2018, 23(5), 1004; https://doi.org/10.3390/molecules23051004

A Metabolism-Based Synergy for Total Coumarin Extract of Radix Angelicae Dahuricae and Ligustrazine on Migraine Treatment in Rats

1
College of Pharmaceutical Sciences and Chinese Medicine, Southwest University, 2# Tiansheng Road, Beibei District, Chongqing 400715, China
2
School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, 312# Anshanxi Road, Nankai District, Tianjin 300193, China
3
Tianjin State Key Laboratory of Modern Chinese Medicine, 88# Yuquan Road, Nankai District, Tianjin 300193, China
4
Research Institute for Liver Diseases (Shanghai) Co. Ltd., 200# Niudun Road, Shanghai 201203, China
*
Author to whom correspondence should be addressed.
Received: 21 March 2018 / Revised: 19 April 2018 / Accepted: 21 April 2018 / Published: 25 April 2018
(This article belongs to the Section Natural Products Chemistry)
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Abstract

Radix Angelicae dahuricae, containing coumarins, which might affect cytochrome P450 enzyme (CYP450) activity, has been co-administered with ligustrazine, a substrate of CYP450s, for the clinical treatment of migraine. However, whether a pharmacokinetic-based synergy exists between Radix Angelicae dahuricae and ligustrazine is still unknown. In this study, the total coumarin extract (TCE) of Radix Angelicae dahuricae (50 mg/kg, orally) reinforced the anti-migraine activity of ligustrazine by declining head scratching, plasma calcitonin gene-related peptide, and serum nitric oxide, as well as increasing plasma endothelin levels in rats (p < 0.05). Moreover, the pharmacokinetic study reflected that TCE potentiated the area under the concentration–time curve of ligustrazine and prolonged its mean retention time in rats (p < 0.05). Besides, the IC50 for TCE, imperatorin and isoimperatorin inhibiting ligustrazine metabolism were 5.0 ± 1.02, 1.35 ± 0.46, 4.81 ± 1.14 µg/mL in human liver microsomes, and 13.69 ± 1.11, 1.19 ± 1.09, 1.69 ± 1.17 µg/mL in rat liver microsomes, respectively. Moreover, imperatorin and isoimperatorin were CYP450s inhibitors with IC50 < 10 µM for CYP1A2, 2C9, 2D6, and 3A4. Therefore, this study concluded that Radix Angelicae dahuricae could increase ligustrazine plasma concentration and then reinforce its pharmacological effect by inhibiting its metabolism through interference with CYP450s. This could be one mechanism for the synergy between Radix Angelicae dahuricae and ligustrazine on migraine treatment. View Full-Text
Keywords: CYP 450s; ligustrazine; migraine; Radix Angelicae dahuricae; synergy CYP 450s; ligustrazine; migraine; Radix Angelicae dahuricae; synergy
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    Description: method validation results of ligustrazine, hydroxy-ligustrazine, imperatorin and isoimperatorin,
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Feng, S.; He, X.; Zhong, P.; Zhao, J.; Huang, C.; Hu, Z. A Metabolism-Based Synergy for Total Coumarin Extract of Radix Angelicae Dahuricae and Ligustrazine on Migraine Treatment in Rats. Molecules 2018, 23, 1004.

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