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Molecules 2018, 23(4), 811; https://doi.org/10.3390/molecules23040811

Combination of Superheated Steam with Laccase Pretreatment Together with Size Reduction to Enhance Enzymatic Hydrolysis of Oil Palm Biomass

1
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia
2
Laboratory of Biopolymer and Derivatives, Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia
*
Author to whom correspondence should be addressed.
Received: 22 February 2018 / Revised: 20 March 2018 / Accepted: 23 March 2018 / Published: 2 April 2018
(This article belongs to the Special Issue Efficient Technology for the Pretreatment of Biomass)
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Abstract

The combination of superheated steam (SHS) with ligninolytic enzyme laccase pretreatment together with size reduction was conducted in order to enhance the enzymatic hydrolysis of oil palm biomass into glucose. The oil palm empty fruit bunch (OPEFB) and oil palm mesocarp fiber (OPMF) were pretreated with SHS and ground using a hammer mill to sizes of 2, 1, 0.5 and 0.25 mm before pretreatment using laccase to remove lignin. This study showed that reduction of size from raw to 0.25 mm plays important role in lignin degradation by laccase that removed 38.7% and 39.6% of the lignin from OPEFB and OPMF, respectively. The subsequent saccharification process of these pretreated OPEFB and OPMF generates glucose yields of 71.5% and 63.0%, which represent a 4.6 and 4.8-fold increase, respectively, as compared to untreated samples. This study showed that the combination of SHS with laccase pretreatment together with size reduction could enhance the glucose yield. View Full-Text
Keywords: oil palm biomass; physical pretreatment; biological pretreatment; lignin removal; lignocellulosic biomass oil palm biomass; physical pretreatment; biological pretreatment; lignin removal; lignocellulosic biomass
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Ahmad Rizal, N.F.A.; Ibrahim, M.F.; Zakaria, M.R.; Kamal Bahrin, E.; Abd-Aziz, S.; Hassan, M.A. Combination of Superheated Steam with Laccase Pretreatment Together with Size Reduction to Enhance Enzymatic Hydrolysis of Oil Palm Biomass. Molecules 2018, 23, 811.

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