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Mechano-Enzymatic Deconstruction with a New Enzymatic Cocktail to Enhance Enzymatic Hydrolysis and Bioethanol Fermentation of Two Macroalgae Species

1
UMR, Ingénierie des Agropolymères et des Technologies Emergentes (IATE), CIRAD, Montpellier SupAgro, INRA, Université de Montpellier, 34060 Montpellier, France
2
APESA, Plateau Technique, Cap Ecologia, Avenue Fréderic Joliot Curie, 64230 Lescar, France
3
AgroBioSciences, Mohammed VI Polytechnic University, Lot 660-Hay Moulay Rachid, Ben Guerir 43150, Morocco
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(1), 174; https://doi.org/10.3390/molecules23010174
Received: 14 November 2017 / Revised: 19 December 2017 / Accepted: 7 January 2018 / Published: 17 January 2018
(This article belongs to the Special Issue Efficient Technology for the Pretreatment of Biomass)
The aim of this study was to explore the efficiency of a mechano-enzymatic deconstruction of two macroalgae species for sugars and bioethanol production, by using a new enzymatic cocktail (Haliatase) and two types of milling modes (vibro-ball: VBM and centrifugal milling: CM). By increasing the enzymatic concentration from 3.4 to 30 g/L, the total sugars released after 72 h of hydrolysis increased (from 6.7 to 13.1 g/100 g TS and from 7.95 to 10.8 g/100 g TS for the green algae U. lactuca and the red algae G. sesquipedale, respectively). Conversely, total sugars released from G. sesquipedale increased (up to 126% and 129% after VBM and CM, respectively). The best bioethanol yield (6 geth/100 g TS) was reached after 72 h of fermentation of U. lactuca and no increase was obtained after centrifugal milling. The latter led to an enhancement of the ethanol yield of G. sesquipedale (from 2 to 4 g/100 g TS). View Full-Text
Keywords: bioethanol; enzymatic hydrolysis; macroalgae; mechanical pretreatment bioethanol; enzymatic hydrolysis; macroalgae; mechanical pretreatment
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MDPI and ACS Style

Amamou, S.; Sambusiti, C.; Monlau, F.; Dubreucq, E.; Barakat, A. Mechano-Enzymatic Deconstruction with a New Enzymatic Cocktail to Enhance Enzymatic Hydrolysis and Bioethanol Fermentation of Two Macroalgae Species. Molecules 2018, 23, 174.

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