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Molecules 2018, 23(1), 174; https://doi.org/10.3390/molecules23010174

Mechano-Enzymatic Deconstruction with a New Enzymatic Cocktail to Enhance Enzymatic Hydrolysis and Bioethanol Fermentation of Two Macroalgae Species

1
UMR, Ingénierie des Agropolymères et des Technologies Emergentes (IATE), CIRAD, Montpellier SupAgro, INRA, Université de Montpellier, 34060 Montpellier, France
2
APESA, Plateau Technique, Cap Ecologia, Avenue Fréderic Joliot Curie, 64230 Lescar, France
3
AgroBioSciences, Mohammed VI Polytechnic University, Lot 660-Hay Moulay Rachid, Ben Guerir 43150, Morocco
*
Author to whom correspondence should be addressed.
Received: 14 November 2017 / Revised: 19 December 2017 / Accepted: 7 January 2018 / Published: 17 January 2018
(This article belongs to the Special Issue Efficient Technology for the Pretreatment of Biomass)
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Abstract

The aim of this study was to explore the efficiency of a mechano-enzymatic deconstruction of two macroalgae species for sugars and bioethanol production, by using a new enzymatic cocktail (Haliatase) and two types of milling modes (vibro-ball: VBM and centrifugal milling: CM). By increasing the enzymatic concentration from 3.4 to 30 g/L, the total sugars released after 72 h of hydrolysis increased (from 6.7 to 13.1 g/100 g TS and from 7.95 to 10.8 g/100 g TS for the green algae U. lactuca and the red algae G. sesquipedale, respectively). Conversely, total sugars released from G. sesquipedale increased (up to 126% and 129% after VBM and CM, respectively). The best bioethanol yield (6 geth/100 g TS) was reached after 72 h of fermentation of U. lactuca and no increase was obtained after centrifugal milling. The latter led to an enhancement of the ethanol yield of G. sesquipedale (from 2 to 4 g/100 g TS). View Full-Text
Keywords: bioethanol; enzymatic hydrolysis; macroalgae; mechanical pretreatment bioethanol; enzymatic hydrolysis; macroalgae; mechanical pretreatment
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Amamou, S.; Sambusiti, C.; Monlau, F.; Dubreucq, E.; Barakat, A. Mechano-Enzymatic Deconstruction with a New Enzymatic Cocktail to Enhance Enzymatic Hydrolysis and Bioethanol Fermentation of Two Macroalgae Species. Molecules 2018, 23, 174.

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