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Molecules 2018, 23(3), 636; https://doi.org/10.3390/molecules23030636

Lung Cancer Chemopreventive Activity of Patulin Isolated from Penicillium vulpinum

1
School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1 Rue Michel-Servet, CH-1211 Geneva 4, Switzerland
2
Mycology and Biotechnology group, Plant, Agroscope, Route de Duillier 60, P.O. Box 1012, 1260 Nyon, Switzerland
*
Author to whom correspondence should be addressed.
Received: 30 January 2018 / Revised: 5 March 2018 / Accepted: 7 March 2018 / Published: 12 March 2018
(This article belongs to the Special Issue Plant Derived Natural Products and Age Related Diseases)
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Abstract

Lung cancer is the most lethal form of cancer in the world. Its development often involves an overactivation of the nuclear factor kappa B (NF-κB) pathway, leading to increased cell proliferation, survival, mobility, and a decrease in apoptosis. Therefore, NF-κB inhibitors are actively sought after for both cancer chemoprevention and therapy, and fungi represent an interesting unexplored reservoir for such molecules. The aim of the present work was to find naturally occurring lung cancer chemopreventive compounds by investigating the metabolites of Penicillium vulpinum, a fungus that grows naturally on dung. Penicillium vulpinum was cultivated in Potato Dextrose Broth and extracted with ethyl acetate. Bioassay-guided fractionation of this extract was performed by measuring NF-κB activity using a HEK293 cell line transfected with an NF-κB-driven luciferase reporter gene. The mycotoxin patulin was identified as a nanomolar inhibitor of TNF-α-induced NF-κB activity. Immunocytochemistry and Western blot analyses revealed that its mechanism of action involved an inhibition of p65 nuclear translocation and was independent from the NF-κB inhibitor α (IκBα) degradation process. Enhancing its interest in lung cancer chemoprevention, patulin also exhibited antiproliferative, proapoptotic, and antimigration effects on human lung adenocarcinoma cells through inhibition of the Wnt pathway. View Full-Text
Keywords: cancer chemoprevention; NF-κB; patulin; Penicillium vulpinum; Wnt cancer chemoprevention; NF-κB; patulin; Penicillium vulpinum; Wnt
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Monteillier, A.; Allard, P.-M.; Gindro, K.; Wolfender, J.-L.; Cuendet, M. Lung Cancer Chemopreventive Activity of Patulin Isolated from Penicillium vulpinum. Molecules 2018, 23, 636.

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