Lung Cancer Chemopreventive Activity of Patulin Isolated from Penicillium vulpinum
Abstract
:1. Introduction
2. Results
2.1. Patulin Isolation through Bioassay-Guided Fractionation
2.2. Patulin Inhibited NF-κB p65 Nuclear Translocation
2.3. Patulin Triggered Apoptotic Cell Death in A549 Cells
2.4. Patulin Inhibited A549 Cell Migration
2.5. Patulin Inhibited the Wnt Pathway
3. Discussion
4. Materials and Methods
4.1. Fungal Culture
4.2. Extraction and Isolation of Patulin
4.3. NMR and HRMS Measurements
4.4. Cell Culture
4.5. Measure of NF-κB Activity
4.6. Immunocytochemistry
4.7. Western Blot Analysis
4.8. Cell Viability Assay
4.9. Apoptosis Assay
4.10. Cell Migration Assay
4.11. Quantitative Real Time PCR Analysis of mRNA Expression
4.12. Statistical Analysis
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Monteillier, A.; Allard, P.-M.; Gindro, K.; Wolfender, J.-L.; Cuendet, M. Lung Cancer Chemopreventive Activity of Patulin Isolated from Penicillium vulpinum. Molecules 2018, 23, 636. https://doi.org/10.3390/molecules23030636
Monteillier A, Allard P-M, Gindro K, Wolfender J-L, Cuendet M. Lung Cancer Chemopreventive Activity of Patulin Isolated from Penicillium vulpinum. Molecules. 2018; 23(3):636. https://doi.org/10.3390/molecules23030636
Chicago/Turabian StyleMonteillier, Aymeric, Pierre-Marie Allard, Katia Gindro, Jean-Luc Wolfender, and Muriel Cuendet. 2018. "Lung Cancer Chemopreventive Activity of Patulin Isolated from Penicillium vulpinum" Molecules 23, no. 3: 636. https://doi.org/10.3390/molecules23030636