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Open AccessArticle

Effect of Aspergillus flavus Fungal Elicitor on the Production of Terpenoid Indole Alkaloids in Catharanthus roseus Cambial Meristematic Cells

by Chuxin Liang 1,†, Chang Chen 2,†, Pengfei Zhou 3,†, Lv Xu 1, Jianhua Zhu 1,2,*, Jincai Liang 2, Jiachen Zi 2 and Rongmin Yu 1,2,*
1
Biotechnological Institute of Chinese Materia Medica, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China
2
Department of Natural Product Chemistry, College of Pharmacy, Jinan University, Guangzhou 510632, China
3
Department of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Molecules 2018, 23(12), 3276; https://doi.org/10.3390/molecules23123276
Received: 22 October 2018 / Revised: 7 December 2018 / Accepted: 10 December 2018 / Published: 11 December 2018
(This article belongs to the Special Issue Advances in Plant Alkaloid Research)
This study reported the inducing effect of Aspergillus flavus fungal elicitor on biosynthesis of terpenoid indole alkaloids (TIAs) in Catharanthus roseus cambial meristematic cells (CMCs) and its inducing mechanism. According to the results determined by HPLC and HPLC-MS/MS, the optimal condition of the A. flavus elicitor was as follows: after suspension culture of C. roseus CMCs for 6 day, 25 mg/L A. flavus mycelium elicitor were added, and the CMC suspensions were further cultured for another 48 h. In this condition, the contents of vindoline, catharanthine, and ajmaline were 1.45-, 3.29-, and 2.14-times as high as those of the control group, respectively. Transcriptome analysis showed that D4H, G10H, GES, IRS, LAMT, SGD, STR, TDC, and ORCA3 were involved in the regulation of this induction process. The results of qRT-PCR indicated that the increasing accumulations of vindoline, catharanthine, and ajmaline in C. roseus CMCs were correlated with the increasing expression of the above genes. Therefore, A. flavus fungal elicitor could enhance the TIA production of C. roseus CMCs, which might be used as an alternative biotechnological resource for obtaining bioactive alkaloids. View Full-Text
Keywords: Catharanthus roseus; cambial meristematic cells; Aspergillus flavus; terpenoid indole alkaloids; biosynthesis Catharanthus roseus; cambial meristematic cells; Aspergillus flavus; terpenoid indole alkaloids; biosynthesis
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MDPI and ACS Style

Liang, C.; Chen, C.; Zhou, P.; Xu, L.; Zhu, J.; Liang, J.; Zi, J.; Yu, R. Effect of Aspergillus flavus Fungal Elicitor on the Production of Terpenoid Indole Alkaloids in Catharanthus roseus Cambial Meristematic Cells. Molecules 2018, 23, 3276.

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