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Open AccessArticle

Metabolomic Analysis of Pollen Grains with Different Germination Abilities from Two Clones of Chinese Fir (Cunninghamia lanceolata (Lamb) Hook)

by Seif Aldin Dawina Abdallah Fragallah 1,2, Pei Wang 1,3, Nuo Li 1,4, Yu Chen 1,3,4,* and Sizu Lin 1,3,4,*
1
College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
Faculty of Natural Resources and Environmental Studies, University of Kordofan, Elobied 160, Sudan
3
State Forestry Administration Engineering Research Center of Chinese Fir, Fuzhou 350002, China
4
Key Laboratory for Forest Adversity Physiological Ecology and Molecular Biology, Fuzhou 350002, China
*
Authors to whom correspondence should be addressed.
Molecules 2018, 23(12), 3162; https://doi.org/10.3390/molecules23123162
Received: 8 October 2018 / Revised: 13 November 2018 / Accepted: 29 November 2018 / Published: 30 November 2018
Pollen grains produce certain metabolites, which can improve or inhibit germination and tube growth. Metabolomic analysis of germinating and growing Chinese fir pollen has not been reported. Therefore, this study aimed to analyse metabolites changes, content and expression in the germinating pollen of Chinese fir. To understand the metabolic differences, two clones from Chinese fir were selected. Metabolomics analyses were performed on three stages (1-, 24- and 48-h) during in vitro pollen germination. The metabolites profiles at different time points were analyzed by using liquid chromatography-mass spectrometry. The results showed that 171 peaks were screened; the corresponding differential metabolites of 121 peaks were classified into nine types of substances. The expression of metabolites showed significant differences across and between clones, and the variation was evident at all germination stages. The expression was obvious at the early stage of germination, which differed clearly from that of the late stage after pollen tube growth. Moreover, the metabolites were mainly enriched in 14 metabolic pathways. Pollen germination and tube growth and metabolites expressions changed per incubation time. Since this work is preliminary, we suggest further investigations to understand the relationship between the differential metabolites and pollen development, and factors affecting pollen germination process. View Full-Text
Keywords: Chinese fir; metabolomics; in-vitro pollen germination; metabolic differences Chinese fir; metabolomics; in-vitro pollen germination; metabolic differences
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Fragallah, S.A.D.A.; Wang, P.; Li, N.; Chen, Y.; Lin, S. Metabolomic Analysis of Pollen Grains with Different Germination Abilities from Two Clones of Chinese Fir (Cunninghamia lanceolata (Lamb) Hook). Molecules 2018, 23, 3162.

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