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Molecules 2018, 23(11), 2944; https://doi.org/10.3390/molecules23112944

Radiobiological Characterization of 64CuCl2 as a Simple Tool for Prostate Cancer Theranostics

1
Centro de Ciências e Tecnologias Nucleares, Instituto Superior Técnico, Universidade de Lisboa, Estrada Nacional 10 (km 139,7), 2695-066 Bobadela LRS, Portugal
2
Instituto de Ciências Nucleares Aplicadas à Saúde, Universidade de Coimbra, Pólo de Ciências da Saúde, Az. Sta. Comba, 3000-548 Coimbra, Portugal
*
Author to whom correspondence should be addressed.
Academic Editors: Licia Uccelli, Alessandra Boschi and Petra Martini
Received: 29 October 2018 / Revised: 7 November 2018 / Accepted: 9 November 2018 / Published: 11 November 2018
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Abstract

64CuCl2 has recently been proposed as a promising agent for prostate cancer (PCa) theranostics, based on preclinical studies in cellular and animal models, and on the increasing number of human studies documenting its use for PCa diagnosis. Nevertheless, the use of 64CuCl2 raises important radiobiological questions that have yet to be addressed. In this work, using a panel of PCa cell lines in comparison with a non-tumoral prostate cell line, we combined cytogenetic approaches with radiocytotoxicity assays to obtain significant insights into the cellular consequences of exposure to 64CuCl2. PCa cells were found to exhibit increased 64CuCl2 uptake, which could not be attributed to increased expression of the main copper cellular importer, hCtr1, as had been previously suggested. Early DNA damage and genomic instability were also higher in PCa cells, with the tumoral cell lines exhibiting deficient DNA-damage repair upon exposure to 64CuCl2. This was corroborated by the observation that 64CuCl2 was more cytotoxic in PCa cells than in non-tumoral cells. Overall, we showed for the first time that PCa cells had a higher sensitivity to 64CuCl2 than healthy cells, supporting the idea that this compound deserved to be further evaluated as a theranostic agent in PCa. View Full-Text
Keywords: copper-64 chloride; theranostics; prostate cancer; radiobiology; auger therapy copper-64 chloride; theranostics; prostate cancer; radiobiology; auger therapy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Guerreiro, J.F.; Alves, V.; Abrunhosa, A.J.; Paulo, A.; Gil, O.M.; Mendes, F. Radiobiological Characterization of 64CuCl2 as a Simple Tool for Prostate Cancer Theranostics. Molecules 2018, 23, 2944.

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