Next Article in Journal
A Turn-On Detection of DNA Sequences by Means of Fluorescence of DNA-Templated Silver Nanoclusters via Unique Interactions of a Hydrated Ionic Liquid
Previous Article in Journal
Optimization of the Synthesis of Glycerol Derived Monoethers from Glycidol by Means of Heterogeneous Acid Catalysis
Article Menu

Export Article

Open AccessArticle
Molecules 2018, 23(11), 2888; https://doi.org/10.3390/molecules23112888

Immobilized Cells of Bacillus circulans ATCC 21783 on Palm Curtain for Fermentation in 5 L Fermentation Tanks

1,2,* , 1,2
,
1,2
,
1,2
,
1,2
,
1,2,3
,
1,2
and
1,2,3
1
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
2
Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China
3
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
*
Author to whom correspondence should be addressed.
Received: 10 October 2018 / Revised: 30 October 2018 / Accepted: 31 October 2018 / Published: 6 November 2018
Full-Text   |   PDF [2842 KB, uploaded 6 November 2018]   |  

Abstract

Palm curtain was selected as carrier to immobilize Bacillus circulans ATCC 21783 to produce β-cyclodextrin (β-CD). The influence for immobilization to CGTase activity was analyzed to determine the operation stability. 83.5% cyclodextrin glycosyltransferases (CGTase) of the 1st cycle could be produced in the 7th cycle for immobilized cells, while only 28.90% CGTase was produced with free cells. When palm curtain immobilized cells were reused at the 2th cycle, enzyme activities were increased from 5003 to 5132 U/mL, which was mainly due to physical adsorption of cells on palm curtain with special concave surface structure. Furthermore, conditions for expanded culture of immobilized cells in a 5 L fermentation tank were optimized through specific rotation speed procedure (from 350 r/min to 450 r/min with step size of 50 r/min) and fixed ventilation capacity (4.5 L/min), relations between biomass, enzyme activity, pH, and oxygen dissolution was investigated, and the fermentation periods under the two conditions were both 4 h shorter. Compared with free cell, immobilized cell was more stable, effective, and had better application potential in industries. View Full-Text
Keywords: cell immobilization; palm curtain; CGTase; cyclodextrin; fermentation cell immobilization; palm curtain; CGTase; cyclodextrin; fermentation
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Wang, J.; Hu, Y.; Qiu, C.; Fan, H.; Yue, Y.; Jiao, A.; Xu, X.; Jin, Z. Immobilized Cells of Bacillus circulans ATCC 21783 on Palm Curtain for Fermentation in 5 L Fermentation Tanks. Molecules 2018, 23, 2888.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top