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Molecules 2018, 23(11), 2874;

Study of Osteocyte Behavior by High-Resolution Intravital Imaging Following Photo-Induced Ischemia

Department of Neurosurgery, Mayo Clinic College of Medicine, 4500 San Pablo road, Jacksonville, FL 32224, USA
Shanghai Medical Schools, Fudan University, 138 Yixueyuan Road, Xuhui, Shanghai 200032, China
Department of Radiation Oncology, UT Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75235, USA
Author to whom correspondence should be addressed.
Academic Editors: Lesheng Teng, Zhaogang Yang and Jing Xie
Received: 14 September 2018 / Revised: 26 October 2018 / Accepted: 28 October 2018 / Published: 4 November 2018
(This article belongs to the Special Issue Application of Novel Method in Pharmaceutical Study)
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Ischemic injuries and local hypoxia can result in osteocytes dysfunction and play a key role in the pathogenesis of avascular osteonecrosis. Conventional imaging techniques including magnetic resonance imaging (MRI) and computed tomography (CT) can reveal structural and functional changes within bony anatomy; however, characterization of osteocyte behavioral dynamics in the setting of osteonecrosis at the single cell resolution is limited. Here, we demonstrate an optical approach to study real-time osteocyte functions in vivo. Using nicotinamide adenine dinucleotide (NADH) as a biomarker for metabolic dynamics in osteocytes, we showed that NADH level within osteocytes transiently increase significantly after local ischemia through non-invasive photo-induced thrombosis of afferent arterioles followed by a steady decline. Our study presents a non-invasive optical approach to study osteocyte behavior through the modulation of local environmental conditions. Thus it provides a powerful toolkit to study cellular processes involved in bone pathologies in vivo. View Full-Text
Keywords: osteocyte; osteonecrosis; two-photon; NADH osteocyte; osteonecrosis; two-photon; NADH

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Yuan, H.; Jiang, W.; Chen, Y.; Kim, B.Y.S. Study of Osteocyte Behavior by High-Resolution Intravital Imaging Following Photo-Induced Ischemia. Molecules 2018, 23, 2874.

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