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Molecules 2018, 23(11), 2874; https://doi.org/10.3390/molecules23112874

Study of Osteocyte Behavior by High-Resolution Intravital Imaging Following Photo-Induced Ischemia

1
Department of Neurosurgery, Mayo Clinic College of Medicine, 4500 San Pablo road, Jacksonville, FL 32224, USA
2
Shanghai Medical Schools, Fudan University, 138 Yixueyuan Road, Xuhui, Shanghai 200032, China
3
Department of Radiation Oncology, UT Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75235, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Lesheng Teng, Zhaogang Yang and Jing Xie
Received: 14 September 2018 / Revised: 26 October 2018 / Accepted: 28 October 2018 / Published: 4 November 2018
(This article belongs to the Special Issue Application of Novel Method in Pharmaceutical Study)
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Abstract

Ischemic injuries and local hypoxia can result in osteocytes dysfunction and play a key role in the pathogenesis of avascular osteonecrosis. Conventional imaging techniques including magnetic resonance imaging (MRI) and computed tomography (CT) can reveal structural and functional changes within bony anatomy; however, characterization of osteocyte behavioral dynamics in the setting of osteonecrosis at the single cell resolution is limited. Here, we demonstrate an optical approach to study real-time osteocyte functions in vivo. Using nicotinamide adenine dinucleotide (NADH) as a biomarker for metabolic dynamics in osteocytes, we showed that NADH level within osteocytes transiently increase significantly after local ischemia through non-invasive photo-induced thrombosis of afferent arterioles followed by a steady decline. Our study presents a non-invasive optical approach to study osteocyte behavior through the modulation of local environmental conditions. Thus it provides a powerful toolkit to study cellular processes involved in bone pathologies in vivo. View Full-Text
Keywords: osteocyte; osteonecrosis; two-photon; NADH osteocyte; osteonecrosis; two-photon; NADH
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Yuan, H.; Jiang, W.; Chen, Y.; Kim, B.Y.S. Study of Osteocyte Behavior by High-Resolution Intravital Imaging Following Photo-Induced Ischemia. Molecules 2018, 23, 2874.

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