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Molecules 2018, 23(11), 2852; https://doi.org/10.3390/molecules23112852

Exogenous Melatonin Mitigates Methyl Viologen-Triggered Oxidative Stress in Poplar Leaf

1,†
,
1,2,†
and
1,3,*
1
College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi, China
2
Guizhou Academy of Forestry, Guiyang 550005, Guizhou, China
3
Qinling National Forest Ecosystem Research Station, Huoditang, Ningshan 711600, Shaanxi, China
These authors equally contributed to this work.
*
Author to whom correspondence should be addressed.
Received: 11 October 2018 / Revised: 1 November 2018 / Accepted: 1 November 2018 / Published: 2 November 2018
(This article belongs to the Section Bioorganic Chemistry)
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Abstract

As a ubiquitous molecule, melatonin plays a crucial role in tolerance to multiple stresses in plants. In the present work, we report the role of exogenous melatonin in relieving oxidative stress induced by methyl viologen (MV) in poplar (Populus alba × Populus glandulosa) leaf. Leaf discs pretreated with melatonin exhibited increased tolerance to MV-mediated oxidative stress. It was observed that melatonin pretreatment effectively reduced membrane damage and lipid oxidation as demonstrated by decreased relative electrolyte leakage and malonaldehyde content in poplar leaf discs. Exogenous melatonin also stimulated activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX), and enhanced accumulation of non-enzymatic antioxidants of AsA and GSH in leaf discs exposed to MV. In addition, pretreatment of melatonin prompted expression of genes for those antioxidant enzymes. Notably, exogenous melatonin increased expression of P5CS, a key gene for proline biosynthesis, under MV treatment. It was further observed that pretreatment with melatonin boosted activity of P5CS as well as accumulation of proline in leaf discs under MV-mediated oxidative stress. Collectively, this work provides evidence for the ameliorative effect of melatonin on MV-induced oxidative stress in poplar leaf. View Full-Text
Keywords: melatonin; reactive oxygen species; oxidative stress; methyl viologen; proline; poplar melatonin; reactive oxygen species; oxidative stress; methyl viologen; proline; poplar
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Ding, F.; Wang, G.; Zhang, S. Exogenous Melatonin Mitigates Methyl Viologen-Triggered Oxidative Stress in Poplar Leaf. Molecules 2018, 23, 2852.

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