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Article

Formation of α- and β-Cembratriene-Diols in Tobacco (Nicotiana tabacum L.) Is Regulated by Jasmonate-Signaling Components via Manipulating Multiple Cembranoid Synthetic Genes

by
Jinkai Sui
1,
Chunkai Wang
1,
Xiaofeng Liu
1,
Ning Fang
1,
Yanhua Liu
1,
Wenjing Wang
1,
Ning Yan
1,
Huai-Bao Zhang
1,
Yongmei Du
1,
Xinmin Liu
1,
Tiegang Lu
2,
Zhongfeng Zhang
1 and
Hongbo Zhang
1,*
1
Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China
2
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(10), 2511; https://doi.org/10.3390/molecules23102511
Submission received: 19 August 2018 / Revised: 25 September 2018 / Accepted: 28 September 2018 / Published: 30 September 2018
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Abstract

Cembranoids are a group of natural diterpenoid compounds with pharmaceutical potentials, and the cembratriene-diols produced by Nicotiana (tobacco) species display activities in anti-nicotine addiction and neuron protection. Although the enzymes catalyzing cembratriene-diols’ formation in tobacco have been investigated, the regulatory mechanism underlying this physiological process remains unknown. This study has investigated the roles of phytohormone jasmonic acid (JA) in regulating cembratriene-diol formation in N. tabacum cv. TN90 and found that JA and COI1, the receptor protein of the bioactive derivative of JA (i.e., JA-Ile), display critical roles in regulating cembratriene-diols’ formation and the expression of cembranoid synthetic genes CBTS, P450 and NtLTP1. Further studies showed that over-expressing either the gene encoding bHLH transcription factor MYC2a or that encoding MYB transcription factor MYB305 could upregulate the cembranoid synthetic genes and enhance the cembranoid production in plants with dysfunction of COI1. Further studies suggest that COI1 and its downstream regulators MYC2a and MYB305 also modulate the trichome secretion, which is correlated with cembranoid formation. Taken together, this study has demonstrated a critical role of JA-signaling components in governing the cembratriene-diol formation and the transcription of cembratriene-diol synthetic genes in tobacco. Findings in this study are of great importance to reveal the molecular regulatory mechanism underlying cembranoid synthesis.
Keywords: tobacco; cembratriene-diol; secondary metabolism; jasmonate; COI1 tobacco; cembratriene-diol; secondary metabolism; jasmonate; COI1

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MDPI and ACS Style

Sui, J.; Wang, C.; Liu, X.; Fang, N.; Liu, Y.; Wang, W.; Yan, N.; Zhang, H.-B.; Du, Y.; Liu, X.; et al. Formation of α- and β-Cembratriene-Diols in Tobacco (Nicotiana tabacum L.) Is Regulated by Jasmonate-Signaling Components via Manipulating Multiple Cembranoid Synthetic Genes. Molecules 2018, 23, 2511. https://doi.org/10.3390/molecules23102511

AMA Style

Sui J, Wang C, Liu X, Fang N, Liu Y, Wang W, Yan N, Zhang H-B, Du Y, Liu X, et al. Formation of α- and β-Cembratriene-Diols in Tobacco (Nicotiana tabacum L.) Is Regulated by Jasmonate-Signaling Components via Manipulating Multiple Cembranoid Synthetic Genes. Molecules. 2018; 23(10):2511. https://doi.org/10.3390/molecules23102511

Chicago/Turabian Style

Sui, Jinkai, Chunkai Wang, Xiaofeng Liu, Ning Fang, Yanhua Liu, Wenjing Wang, Ning Yan, Huai-Bao Zhang, Yongmei Du, Xinmin Liu, and et al. 2018. "Formation of α- and β-Cembratriene-Diols in Tobacco (Nicotiana tabacum L.) Is Regulated by Jasmonate-Signaling Components via Manipulating Multiple Cembranoid Synthetic Genes" Molecules 23, no. 10: 2511. https://doi.org/10.3390/molecules23102511

APA Style

Sui, J., Wang, C., Liu, X., Fang, N., Liu, Y., Wang, W., Yan, N., Zhang, H.-B., Du, Y., Liu, X., Lu, T., Zhang, Z., & Zhang, H. (2018). Formation of α- and β-Cembratriene-Diols in Tobacco (Nicotiana tabacum L.) Is Regulated by Jasmonate-Signaling Components via Manipulating Multiple Cembranoid Synthetic Genes. Molecules, 23(10), 2511. https://doi.org/10.3390/molecules23102511

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