Next Article in Journal
Hepatoprotective Effect of Ugonin M, A Helminthostachys zeylanica Constituent, on Acetaminophen-Induced Acute Liver Injury in Mice
Next Article in Special Issue
Establishing the Phenolic Composition of Olea europaea L. Leaves from Cultivars Grown in Morocco as a Crucial Step Towards Their Subsequent Exploitation
Previous Article in Journal
Influence of Eugenia uniflora Extract on Adhesion to Human Buccal Epithelial Cells, Biofilm Formation, and Cell Surface Hydrophobicity of Candida spp. from the Oral Cavity of Kidney Transplant Recipients
Previous Article in Special Issue
Natural Scaffolds with Multi-Target Activity for the Potential Treatment of Alzheimer’s Disease
Open AccessFeature PaperArticle

Unravelling the Distribution of Secondary Metabolites in Olea europaea L.: Exhaustive Characterization of Eight Olive-Tree Derived Matrices by Complementary Platforms (LC-ESI/APCI-MS and GC-APCI-MS)

1
Department of Analytical Chemistry, Faculty of Science, University of Granada, Ave. Fuentenueva s/n, 18071 Granada, Spain
2
Bruker Daltonik GmbH, Fahrenheitstraße 4, 28359 Bremen, Germany
3
Acer Campestres S.L. Almendro, 37 (Pol. Ind. El Cerezo), Castillo de Locubín, 23670 Jaén, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Maria Carla Marcotullio
Molecules 2018, 23(10), 2419; https://doi.org/10.3390/molecules23102419
Received: 16 August 2018 / Revised: 12 September 2018 / Accepted: 19 September 2018 / Published: 20 September 2018
In order to understand the distribution of the main secondary metabolites found in Olea europaea L., eight different samples (olive leaf, stem, seed, fruit skin and pulp, as well as virgin olive oil, olive oil obtained from stoned and dehydrated fruits and olive seed oil) coming from a Picudo cv. olive tree were analyzed. All the experimental conditions were selected so as to assure the maximum coverage of the metabolome of the samples under study within a single run. The use of LC and GC with high resolution MS (through different ionization sources, ESI and APCI) and the annotation strategies within MetaboScape 3.0 software allowed the identification of around 150 compounds in the profiles, showing great complementarity between the evaluated methodologies. The identified metabolites belonged to different chemical classes: triterpenic acids and dialcohols, tocopherols, sterols, free fatty acids, and several sub-types of phenolic compounds. The suitability of each platform and polarity (negative and positive) to determine each family of metabolites was evaluated in-depth, finding, for instance, that LC-ESI-MS (+) was the most efficient choice to ionize phenolic acids, secoiridoids, flavonoids and lignans and LC-APCI-MS was very appropriate for pentacyclic triterpenic acids (MS (−)) and sterols and tocopherols (MS (+)). Afterwards, a semi-quantitative comparison of the selected matrices was carried out, establishing their typical features (e.g., fruit skin was pointed out as the matrix with the highest relative amounts of phenolic acids, triterpenic compounds and hydroxylated fatty acids, and seed oil was distinctive for its high relative levels of acetoxypinoresinol and tocopherols). View Full-Text
Keywords: Olea europaea L.; liquid chromatography; gas chromatography; mass spectrometry; secondary metabolites Olea europaea L.; liquid chromatography; gas chromatography; mass spectrometry; secondary metabolites
Show Figures

Graphical abstract

MDPI and ACS Style

Olmo-García, L.; Kessler, N.; Neuweger, H.; Wendt, K.; Olmo-Peinado, J.M.; Fernández-Gutiérrez, A.; Baessmann, C.; Carrasco-Pancorbo, A. Unravelling the Distribution of Secondary Metabolites in Olea europaea L.: Exhaustive Characterization of Eight Olive-Tree Derived Matrices by Complementary Platforms (LC-ESI/APCI-MS and GC-APCI-MS). Molecules 2018, 23, 2419. https://doi.org/10.3390/molecules23102419

AMA Style

Olmo-García L, Kessler N, Neuweger H, Wendt K, Olmo-Peinado JM, Fernández-Gutiérrez A, Baessmann C, Carrasco-Pancorbo A. Unravelling the Distribution of Secondary Metabolites in Olea europaea L.: Exhaustive Characterization of Eight Olive-Tree Derived Matrices by Complementary Platforms (LC-ESI/APCI-MS and GC-APCI-MS). Molecules. 2018; 23(10):2419. https://doi.org/10.3390/molecules23102419

Chicago/Turabian Style

Olmo-García, Lucía; Kessler, Nikolas; Neuweger, Heiko; Wendt, Karin; Olmo-Peinado, José M.; Fernández-Gutiérrez, Alberto; Baessmann, Carsten; Carrasco-Pancorbo, Alegría. 2018. "Unravelling the Distribution of Secondary Metabolites in Olea europaea L.: Exhaustive Characterization of Eight Olive-Tree Derived Matrices by Complementary Platforms (LC-ESI/APCI-MS and GC-APCI-MS)" Molecules 23, no. 10: 2419. https://doi.org/10.3390/molecules23102419

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop