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Molecules 2018, 23(1), 93; https://doi.org/10.3390/molecules23010093

Exogenous 24-Epibrassinolide Interacts with Light to Regulate Anthocyanin and Proanthocyanidin Biosynthesis in Cabernet Sauvignon (Vitis vinifera L.)

1
College of Enology, Northwest A&F University, Yangling 712100, China
2
Shaanxi Engineering Research Center for Viti-Viniculture, Yangling 712100, China
3
Chateau Changyu Rena Co., Ltd., Xianyang 712000, China
*
Authors to whom correspondence should be addressed.
Received: 2 November 2017 / Revised: 28 December 2017 / Accepted: 29 December 2017 / Published: 9 January 2018
(This article belongs to the Special Issue Advances in Anthocyanin Research 2018)
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Abstract

Anthocyanins and proanthocyanidins (PAs) are crucial factors that affect the quality of grapes and the making of wine, which were stimulated by various stimuli and environment factors (sugar, hormones, light, and temperature). The aim of the study was to investigate the influence of exogenous 24-Epibrassinolide (EBR) and light on the mechanism of anthocyanins and PAs accumulation in grape berries. Grape clusters were sprayed with EBR (0.4 mg/L) under light and darkness conditions (EBR + L, EBR + D), or sprayed with deionized water under light and darkness conditions as controls (L, D), at the onset of veraison. A large amount of anthocyanins accumulated in the grape skins and was measured under EBR + L and L treatments, whereas EBR + D and D treatments severely suppressed anthocyanin accumulation. This indicated that EBR treatment could produce overlay effects under light, in comparison to that in dark. Real-time quantitative PCR analysis indicated that EBR application up-regulated the expression of genes (VvCHI1, VvCHS2, VvCHS3, VvDFR, VvLDOX, VvMYBA1) under light conditions. Under darkness conditions, only early biosynthetic genes of anthocyanin biosynthesis responded to EBR. Furthermore, we also analyzed the expression levels of the BR-regulated transcription factor VvBZR1 (Brassinazole-resistant 1) and light-regulated transcription factor VvHY5 (Elongated hypocotyl 5). Our results suggested that EBR and light had synergistic effects on the expression of genes in the anthocyanin biosynthesis pathway. View Full-Text
Keywords: grape; anthocyanin biosynthesis; VvBZR1; VvHY5; 24-Epibrassinolide grape; anthocyanin biosynthesis; VvBZR1; VvHY5; 24-Epibrassinolide
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Zhou, Y.; Yuan, C.; Ruan, S.; Zhang, Z.; Meng, J.; Xi, Z. Exogenous 24-Epibrassinolide Interacts with Light to Regulate Anthocyanin and Proanthocyanidin Biosynthesis in Cabernet Sauvignon (Vitis vinifera L.). Molecules 2018, 23, 93.

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