Identification of a Novel Vasodilatory Octapeptide from the Skin Secretion of the African Hyperoliid Frog, Kassina senegalensis
Abstract
1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Specimen Biodata and Harvesting of Skin Secretion
4.2. Fractionation of Skin Secretion by Reverse-Phase HPLC
4.3. Rat Tail Artery Smooth Muscle Bioassay
4.4. Structural Characterisation Using MS/MS Fragmentation Sequencing
4.5. Molecular Cloning of WL-8 Amide Biosynthetic Precursor—Encoding cDNA
4.6. Chemical Synthesis of WL-8 Amide
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
MDPI | Multidisciplinary Digital Publishing Institute |
DOAJ | Directory of open access journals |
TLA | Three letter acronym |
cDNA | Complementary DNA |
TRH | Thyrotropin-releasing hormone |
RP-HPLC | Reverse-phase high performance liquid chromatography |
BLAST | Basic Local Alignment Search Tool |
NCBI | National Centre for Biotechnological Information |
MALDI-TOF | Matrix-assisted laser desorption/ionisation, time-of-flight |
MS | mass spectrometry |
MS/MS | Tandem mass spectrometry |
SEM | Standard error of the mean |
TFA | Trifluoracetic acid |
PS | Peptide synthesiser |
LC/MS/MS | Liquid chromatography coupled tandem mass spectrometry |
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Sample Availability: Samples of the compounds are available from the authors. |
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Du, Q.; Wang, H.; Ma, C.; Wu, Y.; Xi, X.; Zhou, M.; Chen, T.; Shaw, C.; Wang, L. Identification of a Novel Vasodilatory Octapeptide from the Skin Secretion of the African Hyperoliid Frog, Kassina senegalensis. Molecules 2017, 22, 1215. https://doi.org/10.3390/molecules22071215
Du Q, Wang H, Ma C, Wu Y, Xi X, Zhou M, Chen T, Shaw C, Wang L. Identification of a Novel Vasodilatory Octapeptide from the Skin Secretion of the African Hyperoliid Frog, Kassina senegalensis. Molecules. 2017; 22(7):1215. https://doi.org/10.3390/molecules22071215
Chicago/Turabian StyleDu, Qiang, Hui Wang, Chengbang Ma, Yue Wu, Xinping Xi, Mei Zhou, Tianbao Chen, Chris Shaw, and Lei Wang. 2017. "Identification of a Novel Vasodilatory Octapeptide from the Skin Secretion of the African Hyperoliid Frog, Kassina senegalensis" Molecules 22, no. 7: 1215. https://doi.org/10.3390/molecules22071215
APA StyleDu, Q., Wang, H., Ma, C., Wu, Y., Xi, X., Zhou, M., Chen, T., Shaw, C., & Wang, L. (2017). Identification of a Novel Vasodilatory Octapeptide from the Skin Secretion of the African Hyperoliid Frog, Kassina senegalensis. Molecules, 22(7), 1215. https://doi.org/10.3390/molecules22071215