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Molecules 2017, 22(7), 1215;

Identification of a Novel Vasodilatory Octapeptide from the Skin Secretion of the African Hyperoliid Frog, Kassina senegalensis

School of Pharmacy, China Medical University, Shenyang 110001, Liaoning, China
Natural Drug Discovery Group, School of Pharmacy, Queen’s University, Belfast BT9 7BL, Northern Ireland, UK
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Received: 5 July 2017 / Revised: 17 July 2017 / Accepted: 19 July 2017 / Published: 19 July 2017
(This article belongs to the Special Issue Bioactive Natural Peptides As A Pipeline For Therapeutics)
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The defensive skin secretions of amphibians continue to be an excellent source of novel biologically-active peptides. Here we report the identification and pharmacological activity of a novel C-terminally amided myotropic octapeptide from the skin secretion of the African hyperoliid frog, Kassina senegalensis. The 8-amino acid peptide has the following primary structure: WMSLGWSL-amide and has a molecular mass of 978 Da. The primary structure and organisation of the biosynthetic precursor of WL-8 amide was successfully deduced from cloned skin secretion-derived cDNA. The open-reading frame encoded a single copy of WL-8, located at the C-terminus. Synthetic WL-8 amide was found to cause relaxation of rat tail artery smooth muscle with an EC50 of 25.98 nM. This peptide is unique in terms of its primary structure and is unlike any other peptide previously isolated from an amphibian source which has been archived in the NCBI database. WL-8 amide thus represents the prototype of a novel family of myotropic peptide from amphibian defensive skin secretions. View Full-Text
Keywords: amphibian; skin secretion; peptide; smooth muscle amphibian; skin secretion; peptide; smooth muscle

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Du, Q.; Wang, H.; Ma, C.; Wu, Y.; Xi, X.; Zhou, M.; Chen, T.; Shaw, C.; Wang, L. Identification of a Novel Vasodilatory Octapeptide from the Skin Secretion of the African Hyperoliid Frog, Kassina senegalensis. Molecules 2017, 22, 1215.

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