Next Article in Journal
Structural Insight into the Recognition of r(UAG) by Musashi-1 RBD2, and Construction of a Model of Musashi-1 RBD1-2 Bound to the Minimum Target RNA
Next Article in Special Issue
The Role of Solvent-Accessible Leu-208 of Cold-Active Pseudomonas fluorescens Strain AMS8 Lipase in Interfacial Activation, Substrate Accessibility and Low-Molecular Weight Esterification in the Presence of Toluene
Previous Article in Journal
N-Heterocyclic Carbene Coinage Metal Complexes of the Germanium-Rich Metalloid Clusters [Ge9R3] and [Ge9RI2]2 with R = Si(iPr)3 and RI = Si(TMS)3
Open AccessArticle

Preparation of Carriers Based on ZnO Nanoparticles Decorated on Graphene Oxide (GO) Nanosheets for Efficient Immobilization of Lipase from Candida rugosa

Hubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Key Laboratory of Oilseeds Processing, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
*
Authors to whom correspondence should be addressed.
Molecules 2017, 22(7), 1205; https://doi.org/10.3390/molecules22071205
Received: 12 May 2017 / Revised: 28 June 2017 / Accepted: 12 July 2017 / Published: 19 July 2017
(This article belongs to the Special Issue Lipases and Lipases Modification)
Herein, a promising carrier, graphene oxide (GO) decorated with ZnO nanoparticles, denoted as GO/ZnO composite, has been designed and constructed. This carrier was characterized by X-ray powder diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy and thermogravimetry. Then, Candida rugosa lipase (CRL) was immobilized onto the GO-based materials via physical adsorption. Our results indicated that the lipase loading amount on the GO/ZnO composites was about 73.52 mg of protein per g. In the activity assay, the novel immobilized lipase GO/[email protected], exhibited particularly excellent performance in terms of thermostability and reusability. Within 30 min at 50 °C, the free lipase, [email protected] and [email protected] had respectively lost 64%, 62% and 41% of their initial activity. However, GO/[email protected] still retained its activity of 63% after 180 min at 50 °C. After reuse of the GO/[email protected] 14 times, 90% of the initial activity can be recovered. Meanwhile, the relative activity of [email protected] and [email protected] was 28% and 23% under uniform conditions. Hence, GO-decorated ZnO nanoparticles may possess great potential as carriers for immobilizing lipase in a wide range of applications. View Full-Text
Keywords: graphene oxide; ZnO nanoparticles; immobilized CRL; enzyme activity; reusability graphene oxide; ZnO nanoparticles; immobilized CRL; enzyme activity; reusability
Show Figures

Graphical abstract

MDPI and ACS Style

Zhang, S.; Shi, J.; Deng, Q.; Zheng, M.; Wan, C.; Zheng, C.; Li, Y.; Huang, F. Preparation of Carriers Based on ZnO Nanoparticles Decorated on Graphene Oxide (GO) Nanosheets for Efficient Immobilization of Lipase from Candida rugosa. Molecules 2017, 22, 1205.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop